Words similar to dtt

Example sentences for: dtt

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Cleavage reactions contained 30 nM substrate RNA, increasing amounts (60 nM) of ribozyme, 20 mM MgCl 2 and 20 mM Tris-HCl (pH 8.0), 1 μl DTT, and 1 μl RNA inhibitor in a final volume of 10 μl.
The transcription reaction mixtures contained lug of linearized DNA; 0.5 mM(each) ATP, CTP, and UTP; 0.1 mM GTP; 0.5 mM cap analog (NEBL); 10 mM DTT; 40 U of Rnasin (Promega); 30 U of SP6 RNA polymerase; and 1 × SP6 RNA polymerase buffer (Promega) in a volume of 30 ul.
Cellular fractionation was performed as described in earlier reports [ 122 123 124 125 ] . Cells were scraped into 100 μl of ice-cold buffer A [10 mM Hepes (pH 7.9); 1.5 mM MgCl 2 ; 10 mM KCl; 0.5 mM DTT; 0.05% NP-40].
Small samples (2 μg) from each RNA fraction were reverse transcribed and radiolabeled (2 μg oligo dT, 1x First Strand buffer, 5 mM DTT, 75 U SuperscriptII [Life Technologies], 20 mM dNTP mix containing dATP, dGTP, dTTP and 100 mCi 33P-dCTP (ICN Radiochemicals).
500 ng (FAM labelling) or 1 μg (JOE and NED labelling) of the cDNA plasmid preparation was mixed with 0.5 pmol labelled oligonucleotides, 0.25 (FAM) or 0.5 (JOE and NED) Units Bgl I, and 10 (FAM) or 20 (JOE and NED) Units T4-DNA-ligase (New England Biolabs), respectively, in 20 mM Tris-acetate (pH 7.9), 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT, 100 μg/ml BSA, 1 mM ATP.