Example sentences for: dtt

How can you use “dtt” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 5 mM DTT was included to inactivate any residual PMSF, and then 2 mM CaCl 2 was added to allow proteolysis of Boi1-PH.

  • To lyse the cells, DTT (SIGMA, The Netherlands) (1 mM) and Triton x-100 (0.

  • The fractions were concentrated by an Ultrafree-15 centrifugal filter device (Millipore Corporation), and loaded onto an FPLC-connected Superdex 75 HR 10/30 (Pharmacia Biotech) column which had been equilibrated with 50 mM phosphate buffer (pH 7.0) containing 1 mM DTT and 150 mM NaCl.

  • All the buffers contained 1 mM DTT.

  • To assemble Cdc14 beads with Net1N for two assays (Figure 4B, detection method: Western blotting), 2 μl of rabbit-anti-GST antibodies were absorbed to 40 μl of protein A beads (Sigma) in 100 μl of HBS [20 mM HEPES (pH 7.2), 150 mM NaCl] + 2 mM EDTA + 0.1 mg/ml BSA + 1 mM DTT (HEBD) buffer for 1 hr.


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