Words similar to dtt

Example sentences for: dtt

How can you use “dtt” in a sentence? Here are some example sentences to help you improve your vocabulary:

at 4°C and 15,000 G. The supernatant was used to analyze cytoplasmatic proteins, nuclei were resuspended in 60 μl of ice cold buffer B [20 mM Hepes (pH 7.9); 25% (v/v) glycerol; 420 mM KCl; 1.5 mM MgCl 2 ; 0.2 mM EDTA; 0.5 mM DTT; 0.5 mM PMSF] and incubated on ice for 15 min.
The beads with the bound proteins were washed and then resuspended in 30 μl of kinase buffer [50 mM HEPES, pH 7.4, 2.5 mM EGTA, 10 mM β-glycerophosphate, 0.1 mM vanadate, 1 mM NaF, 1 mM DTT, 10 mM MgCl 2 , 20 μM ATP, 50 μCi γ- 32P-ATP (7000 Ci/mmol, ICN), and 1 μg histone H1].
Precipitates were washed four times in 20 mM Tris/HCI, pH 7.4; 150 mM NaCl; 0.5% Triton X-100, 10 μg/ml leupeptin, 10 μg/ml pepstatin, 5 μg/ml aprotinin, 1 mM PMSF, and equilibrated in 20 mM HEPES, pH 7.4, 100 mM potassium acetate, 1 mM DTT.
The next two lanes shown are those of oocytes exposed to the reducing agents DTT (100 mM) and TCEP (20 mM) both during processing and in the sample buffer.
To lyse the cells, DTT (SIGMA, The Netherlands) (1 mM) and Triton x-100 (0.