Words similar to dtt

Example sentences for: dtt

How can you use “dtt” in a sentence? Here are some example sentences to help you improve your vocabulary:

To generate labeled single-stranded cDNA target, 10 μg total RNA from rat heart or brain (Clontech, Palo Alto, CA) was reverse transcribed for 2-3 h at 42°C in the presence of 6 μg random primers (Invitrogen/Life Technologies), 1x first-strand synthesis buffer (Invitrogen/Life Technologies), 10 mM DTT, dNTP mix (25 mM dATP, 25 mM dCTP, 25 mM dGTP, 15 mM dTTP, 10 mM amino allyl-dUTP), and 200 units Superscript II reverse transcriptase (Invitrogen/Life Technologies).
The beads were washed twice with HBS + 2 mM DTT + 0.2% Triton and once with HBS + 1 mM DTT.
The final reaction included 1X First Strand Buffer (Life Technologies), 5 mM DTT (Life Technologies), 1.5 mM dATP, dGTP and dTTP, 1.5 μl Superscript II Reverse Transcriptase (Life Technologies), and 10 μl of 33P-dCTP (ICN, specific activity 3,000 Ci/mmol).
All the buffers contained 1 mM DTT.
The RNA/primer mixture was denatured at 70°C for 3 min and cooled on ice for 2 min, followed by adding: 4 μl of 5X first strand buffer (Invitrogen™), 2 μl 0.1 M DTT, 1 μl RNasin ®(40 U/μl, Promega™), 2 μl 10 mM dNTP, and 2 μl Superscript™ II (200 U/μl, Invitrogen™), and incubated at 42°C for 1.5 hours.