Words similar to dtt

Example sentences for: dtt

How can you use “dtt” in a sentence? Here are some example sentences to help you improve your vocabulary:

3 M NaCl, 0.03 M sodium citrate; pH 7.0)/10 mM DTT, treated with RNase A (20 mg/ml) and washed 2 × 30 min at 65°C in 0.1× SSC to remove nonspecific label.
The reaction cocktail contained ~2 μg poly(A) +RNA, 1x reaction buffer (50 mM Tris-HCl pH 8, 75 mM KCl, 3 mM MgCL 2 , 50 μM dNTP, 10 mM DTT), 1 μg oligo dT, 3 μg random hexamer, and 400 units Superscript II (GibcoBRL).
9, 300 mM NaCl, 50 mM KCl, 0.1 mM EDTA, 10% glycerol, 1 mM DTT, and 1 mM PMSF).
P. falciparum (3D7 or Dd2) was grown in human A-positive erythrocytes and infected cells were harvested at about 15% parasitemia and lysed by the saponin method essentially as described [ 14 15 ] . PfPP5 was partially purified using published procedures [ 18 ] . In brief, the parasites were resuspended in buffer A (50 mM Tris-Cl, pH 7.5, 10% glycerol, 1 mM DTT) plus 20 mM NaCl, 1% Triton X-100, leupeptin (1 μg/ml), and aprotinin (1 μg/ml) at an approximate protein concentration of 10 mg per ml.
The purified PCR product (5 μl) was transcribed in 96-well format by incubation for 2 h at 37°C after the addition of 5 μl of a polymerase reaction cocktail consisting of 2 U T7 RNA polymerase, 4.6 U RNase inhibitor, 10 mM NTPs, 3.5 mM digoxigenin-11-UTP, 40 mM Tris pH 8.0, 6 mM MgCl 2 , 10 mM DTT and 2 mM spermidine.