Words similar to dtt

Example sentences for: dtt

How can you use “dtt” in a sentence? Here are some example sentences to help you improve your vocabulary:

Reverse transcription was carried out in a 20 μl volume containing 1 μg total RNA, 1 μg oligdT25- T7 (5'-AAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGG -CGATT-3') primer, 1 μg template switch primer (5'-AAGCAGTGGTAACAACGC -AGGGACCGGG-3'), 4 μl first-strand reaction buffer, 2 μl 10 mM dithiothreitol (DTT; Gibco-BRL) 1 μl 10 mM dNTPs, 1 μl SUPERase-in, (Amtion, Austin, TX) and 200 u Superscript II reverse transcriptase (Gibco- BRL).
The reaction cocktail contained ~2 μg poly(A) +RNA, 1x reaction buffer (50 mM Tris-HCl pH 8, 75 mM KCl, 3 mM MgCL 2 , 50 μM dNTP, 10 mM DTT), 1 μg oligo dT, 3 μg random hexamer, and 400 units Superscript II (GibcoBRL).
The next two lanes shown are those of oocytes exposed to the reducing agents DTT (100 mM) and TCEP (20 mM) both during processing and in the sample buffer.
5 M NaCI/1 mM DTT/1 mM PMSF/leupeptin/aprotinin/600 μM Na 3 VO 4 /50 mM NaF).
PP5 activity was followed during the purification by assaying for trypsin-stimulated phosphohydrolase activity at 37°C vs. p-nitrophenyl phosphate in 200 μl reactions containing: 25 mM tris pH 8.3, 25 mM MgCl 2 , 1 mM DTT and 10 mM p-nitrophenyl phosphate.