Words similar to dtt
Example sentences for: dtt
How can you use “dtt” in a sentence? Here are some example sentences to help you improve your vocabulary:
5 mM DTT was included to inactivate any residual PMSF, and then 2 mM CaCl 2 was added to allow proteolysis of Boi1-PH.
To lyse the cells, DTT (SIGMA, The Netherlands) (1 mM) and Triton x-100 (0.
The fractions were concentrated by an Ultrafree-15 centrifugal filter device (Millipore Corporation), and loaded onto an FPLC-connected Superdex 75 HR 10/30 (Pharmacia Biotech) column which had been equilibrated with 50 mM phosphate buffer (pH 7.0) containing 1 mM DTT and 150 mM NaCl.
All the buffers contained 1 mM DTT.
To assemble Cdc14 beads with Net1N for two assays (Figure 4B, detection method: Western blotting), 2 μl of rabbit-anti-GST antibodies were absorbed to 40 μl of protein A beads (Sigma) in 100 μl of HBS [20 mM HEPES (pH 7.2), 150 mM NaCl] + 2 mM EDTA + 0.1 mg/ml BSA + 1 mM DTT (HEBD) buffer for 1 hr.