Words similar to dtt

Example sentences for: dtt

How can you use “dtt” in a sentence? Here are some example sentences to help you improve your vocabulary:

The reaction cocktail contained ~2 μg poly(A) +RNA, 1x reaction buffer (50 mM Tris-HCl pH 8, 75 mM KCl, 3 mM MgCL 2 , 50 μM dNTP, 10 mM DTT), 1 μg oligo dT, 3 μg random hexamer, and 400 units Superscript II (GibcoBRL).
In some cases, 50 mM Tris-HCl pH 8.0, 2 mM DTT, 100 μg/ml BSA, 50 mM KCl and 8 mM MgCl 2 was used (retroviral reverse transcriptase buffer [ 30]).
After denaturation in 6 M and renaturation in 0.187 M guanidine-HCl in HB buffer (25 mM Hepes pH 7.2, 5 mM NaCl, 5 mM MgCl2, 1 mM DTT), the blots were saturated at 4°C in buffer H (20 mM Hepes pH 7.7, 7.75 mM KCl, 0.1 mM EDTA, 25 mM MgCl 2 , 1 mM DTT, 0.05% NP40, 1% dry milk) for 2 hours, and then incubated for 2 hours at 4°C with 50 μl of 35S-methionine-labeled in vitro-translated hCCR4 or luciferase protein (synthesized using a reticulocyte lysate-coupled transcription/translation kit [Promega]).
During preparation for SDS gel electrophoreses the samples are heated to 72°C in the presence of DTT.
The imidazole-eluted His-tagged PfPP1 was dialyzed against 50 mM Tris-Cl (pH 7.5), 100 mM NaCl, 25% glycerol, 1 mM DTT (buffer A), and stored in small portions at -80°C.