Words similar to dtt

Example sentences for: dtt

How can you use “dtt” in a sentence? Here are some example sentences to help you improve your vocabulary:

Cell lysates for ubiquitylation assays were prepared by hypotonic lysis on ice in 20 mM Hepes pH 7.5, 15 mM MgCl 2 , 5 mM KCl, 1 mM DTT, 10 μg/ml leupeptin, 10 μg/ml pepstatin, 1 mM PMSF, 17 μg/ml aprotinin as described previously [ 30 ] . Lysates were cleared by centrifugation and used without prior freezing.
The imidazole-eluted His-tagged PfPP1 was dialyzed against 50 mM Tris-Cl (pH 7.5), 100 mM NaCl, 25% glycerol, 1 mM DTT (buffer A), and stored in small portions at -80°C.
The PCR product (2 μg in 50 μl) was added to an in vitro transcription reaction containing Ampliscribe T7 RNA polymerase (Epicentre), RNase inhibitor (GIBCO BRL, Rockville, MD; 5 units), low Mg 2+buffer (30 mM Tris-HCl, pH 8, 7.5 mM DTT, 4.5 mM MgCl 2 , 1.5 mM spermidine), UTP (1.
Tissues from ileums, jejunums, and colons were treated with 1 mM DTT (ICN Biomedicals, Aurora, OH) for 30 minutes followed by incubation in calcium/magnesium-free HBSS with EDTA (Life Technologies, Baltimore, MD) 4 times 1 hour with stirring at room temperature to remove the epithelial layer.
Smooth muscle strips were weighed and total protein was extracted by glass-glass homogenization using 50 μl per milligram dry tissue sodium dodecyl sulfate (SDS)-based extraction buffer: 25 mM Tris-HCl, pH 7.4, 2% SDS, 10% glycerol, 1 mM dithiothreitol (DTT), 1 μM leupeptin, 10 mM ethylenediaminetetraacetic acid (EDTA), 1 mM sodium orthovanadate, 5 mM NaF and 1 mM phenylmethylsulfonyl fluoride (PMSF).