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Example sentences for: dpdpe

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Saturation binding assays were carried out for [ 3H]-DPDPE in HEK cells as described previously [ 34 ] . Crude cell homogenate (20-25 μg of per assay tube) was used and incubation was in 50 mM Tris HCl, pH 7.4 at room temperature for 2 hours.
The K d for [ 3H]-DPDPE in these cells was 0.65 ± 0.27 nM.
DPDPE was obtained from Peninsula Laboratories (San Carlos, CA), and [ 3H]-DPDPE was from Multiple Peptide Systems (San Diego, CA).
Binding was carried out in 250 μl volume of 50 mM Tris HCl buffer, pH 7.4 in the presence of 0.5-1 nM [ 3H]-DPDPE and increasing concentrations (24-32 different concentrations) of unlabeled ligand.
At concentrations of 10 μM, neither DSLET, DADLE, nor DPDPE were found to increase the tyrosine phosphorylation of the IR or the PDGFR, whereas insulin (100 ng/ml) or PDGF (50 ng/ml) induced significant tyrosine phosphorylation of these sites (Figure 8).