Words similar to dpdpe

Example sentences for: dpdpe

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DPDPE was obtained from Peninsula Laboratories (San Carlos, CA), and [ 3H]-DPDPE was from Multiple Peptide Systems (San Diego, CA).
One clone expressing 1925 ± 425 fmoles/mg protein, as assessed by [ 3H]-DPDPE binding, was propagated for use in this study.
Saturation binding assays were carried out for [ 3H]-DPDPE in HEK cells as described previously [ 34 ] . Crude cell homogenate (20-25 μg of per assay tube) was used and incubation was in 50 mM Tris HCl, pH 7.4 at room temperature for 2 hours.
Binding was carried out in 250 μl volume of 50 mM Tris HCl buffer, pH 7.4 in the presence of 0.5-1 nM [ 3H]-DPDPE and increasing concentrations (24-32 different concentrations) of unlabeled ligand.
The K d for [ 3H]-DPDPE in these cells was 0.65 ± 0.27 nM.