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Example sentences for: dpab
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Since there are three molecules with strong absorbances at 340 nm (retinal, retinoic acid, and NADH), we developed a high pressure liquid chromatography (HPLC) assay to assess the ability of DPAB to inhibit retinal oxidation by class 1 ALDH.
Cells were exposed to combinations of 1 μM retinoic acid, 1 μM retinal, and various concentrations of DPAB and DEAB prepared in sterile dimethyl sulfoxide (DMSO) or ethanol.
This hypothesis can be tested by using global or specific CYP inhibitors in the presence of retinal in HL-60 cells and by using DPAB in the presence of retinal in an hematopoietic stem cell assay system.
Treatment of L1210/CPA cells in vitro with 50 μM DEAB abolished the tumor cells' resistance to 4-HC [ 19 ] . DEAB also exhibited in vivo efficacy as a ALDH1A1 inhibitor, as demonstrated by the toxicity to the intestinal crypt cells in mice receiving co-injections of DEAB and cyclophosphamide [ 21 ] . More recently, DEAB has been used to demonstrate that overexpression of human ALDH1A1 in transfected cell lines is sufficient to cause cellular resistance to the oxazaphosphorines [ 22 ] and 4-HC [ 23 ] . DEAB has also been an effective reagent in fluorescent-activated cell sorting (FACS) techniques to isolate human hematopoietic stem cells based on the expression of ALDH1A1 activity [ 5 6 ] . In contrast to DEAB and DPAB, daidzin - a natural product isolated from Pueraria lobata (the Kudzu plant) - shows specificity for inhibiting ALDH2 at concentrations similar to that of DPAB used to inhibit ALDH1A1 [ 24 25 ] . An inhibitor specific for ALDH1A2 has not yet been reported.
Since DPAB's inhibition of cell differentiation in cells exposed to retinal was so low, we needed to demonstrate that chemical oxidation or any serum enzymatic oxidation of retinal to retinoic acid was not occuring under our culture conditions.
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