Words similar to dntps
Example sentences for: dntps
How can you use “dntps” in a sentence? Here are some example sentences to help you improve your vocabulary:
The PCR products for primer set cht3(f2) and LTR(r) were amplified in a 25 μl reaction containing Expand Long Template PCR System 10X PCR buffer #1 supplied by Roche (Indianapolis, IN), 0.35 mM dNTPs, 0.32 μM of each primer, and 1.3 U of Expand Long Template PCR System DNA polymerase mix supplied by Roche (Indianapolis, IN).
Six microliters of 5x first-strand buffer (RGI) (1 μl 100 mM DTT, 1.5 μl dNTPs (20 mM), 1.5 μl reverse transcriptase (Superscript II, Gibco BRL), 10 μl [ 33P]dCTP (ICN)) were then added to the primed RNA.
Reaction also contained 200 uM dNTPs, 1 mM Mg ++, and 5 units Taq DNA polymerase (Life Technologies) with supplied reaction buffer.
The PCR reaction mix consisted of 20 mM Tris-HCl (pH 8.4), 50 mM KCl, 0.25 mM each of dNTPs, 2.5 mM MgCl 2 , 0.2 μM each primer, and 0.6 units Tsp polymerase (Platinum GenoTYPE Tsp DNA Polymerase, Cat # 11448-032, Invitrogen, Carlsbad, CA).
The PCR reaction was performed in a final volume of 50 μl using TaqMan Universal PCR Master Mix containing AmpliTaq Gold RDNA Polymerase, AmpErase RUNG, dNTPs (with dUTP), Passive Reference 1, optimized buffer components (proprietary formulation) and 1 μl of cDNA template.
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