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Example sentences for: dntps

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dNTPs, deoxynucleotide triphosphates
Polymerase activity of the HIV-1RT WT and insertion mutant enzymes was determined using two different template-primers: U-5PBS HIV-1 RNA and synthetic 49-mer U5-PBS DNA templates primed with the 17-mer PBS primer [ 36 ] . Assays were carried out in a 50 μL volume containing 50 mM Tris HCl, pH 7.8, 100 μg/mL bovine serum albumin, 5 mM MgCl 2 , 1 mM dithiothreitol, 60 mM KCl, 100 nM template-primer, 50 μM of each of the four dNTPs with one of them being 32P-labeled (0.
The PCR reaction mixture consisted of 1 × buffer A (Perkin-Elmer), 3 mM MgCl 2 , 200 μM dNTPs, 90 nM of each adenovirus primer, 100 nM of each 18S rDNA primer, 200 nM of each probe, 0.6 U AmpliTaq Gold polymerase (Perkin-Elmer) and 5 μl of total DNA sample.
Temperature, pH, cation composition, salt concentration, substrate concentration, and inclusion of ATP and dNTPs were examined.
Similarly, inclusion of 40 μM each of the four dNTPs did not stimulate the RNAseH activity (data not shown).