Words similar to dntps
Example sentences for: dntps
How can you use “dntps” in a sentence? Here are some example sentences to help you improve your vocabulary:
DRF+ RNA and radioactive dNTPs were added, the mixture was incubated at 37°C to allow DNA synthesis, and the products were purified and resolved by denaturing polyacrylamide electrophoresis.
PCR was performed in 50 μL reactions containing: template, 50 pmol each of primers F3 (5'-CATAACGCCAGCCCACCTACTG-3') and ETn1 (5'-CACGATTTGTGGGTAAAATAGGAG C-3'), 2.5 units Taq DNA polymerase, 0.2 mM dNTPs, 20 mM TrisHCl (pH 8.4), 50 mM KCl, and 1.5 mM MgCl.
DNA was amplified in a final reaction volume of 50 μl by using 100 ng genomic DNA, 10x buffer (ABgene Thermo Start AB-0908/b, ABgene, Epson, Surrey, KT19 9AP, U.K.), 1.5 mM MgCl 2 , 200 μM each dNTPs, 0.27 μM(100 ng) primers and 1.25 units Thermo Start polymerase (Abgene).
To generate the probe of HL60 DNA, a PCR reaction was set up with 0.5 μl of the reassociated DNA, 5 μl of 10x Clontech PCR buffer, 1 μl of dNTPS (10 mM dATP, dTTP, and dGTP each, 6 mM dCTP), 4 μl of FluoroLink Cy3-dCTP (Amersham Pharmacia), 4.5 μl of 10 μM CT primer, 1 μl of Clontech cDNA polymerase and 34 μl of ddH 2 O. In the PCR reaction for the probe of HL60/TLK286 DNA, Cy5-dCTP and TT primer were used instead of Cy3-dCTP and CT primer.
Standard RT-PCR reactions were performed with each 50 μL reaction containing 1/8 of an RT reaction, 50 pmol each of forward and reverse primer, 2.5 units Taq DNA polymerase, 0.2 mM dNTPs, 20 mM TrisHCl (pH 8.4), 50 mM KCl, and 1.5 mM MgCl.