Example sentences for: dntps

How can you use “dntps” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Standard RT-PCR reactions were performed with each 50 μL reaction containing 1/8 of an RT reaction, 50 pmol each of forward and reverse primer, 2.5 units Taq DNA polymerase, 0.2 mM dNTPs, 20 mM TrisHCl (pH 8.4), 50 mM KCl, and 1.5 mM MgCl.

  • DNA was amplified in a final reaction volume of 50 μl by using 100 ng genomic DNA, 10x buffer (ABgene Thermo Start AB-0908/b, ABgene, Epson, Surrey, KT19 9AP, U.K.), 1.5 mM MgCl 2 , 200 μM each dNTPs, 0.27 μM(100 ng) primers and 1.25 units Thermo Start polymerase (Abgene).

  • Six microliters of 5x first-strand buffer (RGI) (1 μl 100 mM DTT, 1.5 μl dNTPs (20 mM), 1.5 μl reverse transcriptase (Superscript II, Gibco BRL), 10 μl [ 33P]dCTP (ICN)) were then added to the primed RNA.

  • Taq polymerase, PCR buffer and dNTPs were obtained from QIAGEN and used at recommended concentrations for a 20 ul PCR reaction.

  • The resulting plasmid was then cut with Eco RI, blunt-ended with Klenow polymerase and dNTPs, and self-religated, in order to destroy the Eco RI site at the boundary between core ars3002 and the enhancer that was regenerated during the process of Cla I linker addition.


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