Example sentences for: dntps

How can you use “dntps” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Six microliters first-strand premix were added and incubated at 42°C for 2 h (6 μl mix was made up with 2 μl 5× first-strand buffer, 1 μl 0.1 M DTT, 1 μl dNTPs (10 mM each), 0.3 μl T4gp32 (USB, 13.

  • Reverse transcription was started by adding dNTPs to a final concentration of 1 mM dATP and 500 μM each: dCTP, dGTP, dTTP and 5-(3-aminoallyl)-2'-deoxyuridine-5'-triophosphate, (aa-dUTP) (Sigma), with 150 units of StrataScript (Stratagene, La Jolla, CA).

  • Similarly, inclusion of 40 μM each of the four dNTPs did not stimulate the RNAseH activity (data not shown).

  • dNTPs, deoxynucleotide triphosphates

  • Second strand synthesis and incorporation of the T7 promoter sequence was carried out as follows: the 20 μL tailing reaction product was mixed with 0.6 μL of 25 μM T7-A 18 B primer (5'-GCATTAGCGGCCGCGAAATTAATACGACTCACTATAGGGAG(A) 18 [B], where B refers to C, G or T), 5 μL 10X EcoPol buffer (100 mM Tris-HCl pH 7.5, 50 mM MgCl 2 , 75 mM dTT), 2 μL 5.0 mM dNTPs, and 20.


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