Words similar to dntps
Example sentences for: dntps
How can you use “dntps” in a sentence? Here are some example sentences to help you improve your vocabulary:
Six microliters first-strand premix were added and incubated at 42°C for 2 h (6 μl mix was made up with 2 μl 5× first-strand buffer, 1 μl 0.1 M DTT, 1 μl dNTPs (10 mM each), 0.3 μl T4gp32 (USB, 13.
DRF+ RNA and radioactive dNTPs were added, the mixture was incubated at 37°C to allow DNA synthesis, and the products were purified and resolved by denaturing polyacrylamide electrophoresis.
1) was cut and blunt-ended by treatment with Klenow polymerase (New England Biolabs) and dNTPs.
These oligonucleotides (100 pmole) were annealed and extended with AMV reverse transcriptase (Amersham Pharmacia Biotech, Piscataway, NJ; 45 units) in AMV RT buffer (50 mM Tris-HCl, pH 8.3, 8 mM MgCl 2 , 50 mM NaCl, 1 mM DTT) and dNTPS (200 μM) for 30 minutes at 37°C.
Six microliters of 5x first-strand buffer (RGI) (1 μl 100 mM DTT, 1.5 μl dNTPs (20 mM), 1.5 μl reverse transcriptase (Superscript II, Gibco BRL), 10 μl [ 33P]dCTP (ICN)) were then added to the primed RNA.
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