Words similar to dntps
Example sentences for: dntps
How can you use “dntps” in a sentence? Here are some example sentences to help you improve your vocabulary:
Six microliters first-strand premix were added and incubated at 42°C for 2 h (6 μl mix was made up with 2 μl 5× first-strand buffer, 1 μl 0.1 M DTT, 1 μl dNTPs (10 mM each), 0.3 μl T4gp32 (USB, 13.
Reverse transcription was started by adding dNTPs to a final concentration of 1 mM dATP and 500 μM each: dCTP, dGTP, dTTP and 5-(3-aminoallyl)-2'-deoxyuridine-5'-triophosphate, (aa-dUTP) (Sigma), with 150 units of StrataScript (Stratagene, La Jolla, CA).
Similarly, inclusion of 40 μM each of the four dNTPs did not stimulate the RNAseH activity (data not shown).
dNTPs, deoxynucleotide triphosphates
Second strand synthesis and incorporation of the T7 promoter sequence was carried out as follows: the 20 μL tailing reaction product was mixed with 0.6 μL of 25 μM T7-A 18 B primer (5'-GCATTAGCGGCCGCGAAATTAATACGACTCACTATAGGGAG(A) 18 [B], where B refers to C, G or T), 5 μL 10X EcoPol buffer (100 mM Tris-HCl pH 7.5, 50 mM MgCl 2 , 75 mM dTT), 2 μL 5.0 mM dNTPs, and 20.