Words similar to dntp

Example sentences for: dntp

How can you use “dntp” in a sentence? Here are some example sentences to help you improve your vocabulary:

The E-TP-dNTP ternary complex formation was assessed by the addition of dNTP complementary to the next template base (in this case dGTP, 200 μM).
In this regime, templates in the reaction tube with 1 × PCR buffer and dNTP's were heated to 90°C for 10 minutes then gradually cooled (by approximately 1°C/minute) to 72°C.
For first strand synthesis, RNA (200 ng) in 9 μl water was denatured at 65°C for 4 min, rapidly cooled on ice, and then resuspended in a 20 μl final volume containing 50 mM Tris-HCl pH 8.3, 75 mM KCl, 3 mM MgCl 2 , 10 mM DTT, 1 mM of each dNTP, 20 mM random hexamers and 200 U Superscript II RNase H -RT (GIBCO BRL, Gaithersburg, MD, USA).
To generate labeled single-stranded cDNA target, 10 μg total RNA from rat heart or brain (Clontech, Palo Alto, CA) was reverse transcribed for 2-3 h at 42°C in the presence of 6 μg random primers (Invitrogen/Life Technologies), 1x first-strand synthesis buffer (Invitrogen/Life Technologies), 10 mM DTT, dNTP mix (25 mM dATP, 25 mM dCTP, 25 mM dGTP, 15 mM dTTP, 10 mM amino allyl-dUTP), and 200 units Superscript II reverse transcriptase (Invitrogen/Life Technologies).
Fifty μl PCR reactions contained 1X Perkin Elmer PCR buffer with 1.5 mM MgCl 2 , 0.8 mM each dNTP, 1.25 U AmpliTaq DNA polymerase (Perkin Elmer), and 45 μM of each primer.