Words similar to dntp

Example sentences for: dntp

How can you use “dntp” in a sentence? Here are some example sentences to help you improve your vocabulary:

2 , 0.4 μM TNF-α primer or COX-2 primer, 1:19 ratio of 18S competimers/primers, 0.1 mM each dNTP, 0.2 μl α [ 32P] dCTP (10 mCi/ml; > 3000 Ci/mmol) and 0.025 units of Amplitaq (Perkin Elmer Inc., Norwalk, CT, USA).
In order to determine whether alteration in DNA binding without any change in the apparent dNTP binding affinity of the insertion mutants is consistent with their kinetic parameters, we analyzed the steady-state kinetic parameters of these mutants.
The final amplification reactions contained 10 mM TAPS (pH 8.8), 1.5 mM MgCl 2 , 50 mM KCI, 0.01% gelatin, 0.2 mM each dNTP, 10 pmoles primer, 0.6 U Taq DNA polymerase (Bangalore Genei, Bangalore, India) and 50 ng mulberry DNA template in a 25 μl reaction volume.
RNA (1 μg) was converted to cDNA in a 50 μl reaction containing 1 U of avian myeloblastosis virus reverse transcriptase (New England Biolabs, Beverly, MA, USA), 1 mM dNTP's, and 10 μg/ml random hexamer primers in 1× reverse transcriptase buffer at 42°C for 50 min; the reaction was terminated by heating to 90°C for 10 min.
Specifically, 5 μl of the original 100 μl reaction was transferred to new plates and mixed with 95 μl reaction mix 2 (For 110 reactions; 1.1 ml 10X Buffer, 220 μl 50 mM MgSO 4 , 165 μl premixed 10 mM dNTP's, 550 μl 20 μM universal primer pair, 1.1 ml Pfx Enhancer reagent, 88 μl Pfx enzyme, and 7.227 ml sterile, deionized water).