Words similar to dntp

Example sentences for: dntp

How can you use “dntp” in a sentence? Here are some example sentences to help you improve your vocabulary:

PCR was performed using a standard protocol as follows with a final volume of 50 μl; 5 μl 10X PCR buffer, 5 μl 2 mM dNTP, 5 μl forward primer (concentration 20 ng/ul), 5 ul reverse primer (concentration 20 ng/ul), 1.5 μl 50 mM MgCl, 0.25 μl Taq polymerase (5 U/μl), and 1 μl sample purified genomic DNA (concentration approximately 30 ng/μl) (PCR reagents, Gibco-BRL, Bethesda, MD).
Table 2lists the apparent dNTP binding affinity for the WT enzyme and its insertion mutants determined from data shown in Fig.
PCR amplifications were performed in a 50 μl reaction volume with 60-100 ng genomic DNA, 200 μM of each dNTP and 1.25 units PCR enzyme mix.
An in vitro assay using dideoxy terminated primer annealed with the template which allows the next correct dNTP to bind in the ternary complex without actual DNA synthesis has recently been reported [ 25 ] . Using this assay system, we have evaluated the ability of the insertion mutants to form the ternary complexes and the effect of DNA trap on such complexes.
PCR was run at 50 μM final oligonucleotide primer concentrations and 0.25 mM concentration of each dNTP.