Words similar to dntp

Example sentences for: dntp

How can you use “dntp” in a sentence? Here are some example sentences to help you improve your vocabulary:

Amplification reactions were performed in a total volume of 40 μl and contained 2 μl eluted phage, 40 ng of each primer (FADI 5'-CACTACAATGGATGATG-3' and RADI 5'-CTTGCGGGGTTTTTCAG-3'), 0.001% Tween 20 (Sigma), 2.5 U Taq DNA polymerase (Promega), 1x Taq polymerase buffer (Promega), 1.56 mM MgCl 2 (Promega), and 0.25 mM of each dNTP (USB).
The preformed E-TP complex was then incubated in the presence of next correct dNTP followed by addition of 300-fold molar excess of unlabeled TP as the DNA trap.
For nested amplification, 1 μl of amplified PCR reaction mixture was added to a second PCR reaction mixture (50 μl of 10 mmol/l Tris-HCl [pH 9.0], 50 mmol/l KCl, 1.5 mmol/l MgCl 2 , 0.01% Triton-X, 200 μmol/l dNTP, and 1.25 U Taq DNA polymerase).
Each PCR included 2 μl of the RT reaction containing the primary strand cDNA, reaction buffer [10 mM Tris-HCl (pH 8.4), 50 mM KC1, 1.5 mM MgCl 2 , 0.001% gelatin], 2 μM of each dNTP, 0.2 μM of the same anchored primer used in the RT reaction, 0.2 μM of one of the eight 13-mer arbitrary primers API-8 (Table 1), 12.
The reactions contained 100 ng template DNA, 40 pmoles of primer, 2.5 mM Mg 2+ions, 200 μM each dNTP, 0.3 U Taq DNA polymerase in 1× assay buffer in a final volume of 25 μl.