Example sentences for: dntp

How can you use “dntp” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A sequencing primer was hybridized to the single-stranded copy of the PCR-amplified p53/K- ras 2 fragment and a single base extension with either a Cy-3 or Cy-5 labeled dNTP was performed prior to scanning on a microarray scanner.

  • Since binding of dNTP to the enzyme is an ordered mechanism which occurs only after DNA binding, the extent of labeled TP remaining bound to the enzyme in the presence of dNTP and DNA trap represents the extent of ternary complex formed.

  • Probes were labeled using 10X PCR labeling mix (Boehringer Mannheim, Biochemical Products, Indianapolis IN) at a concentration of 1X replacing the normal dNTP mix.

  • Full-length ds-cDNA was synthesized by adding 57 μl nuclease-free water, 10 μl 10× PCR buffer (Roche), 10 μl 25 mM MgCl 2 , 1 μl 10 mM dNTP and 5 u Ampli-Taq Gold DNA Polymerase (Roche).

  • In order to determine whether alteration in DNA binding without any change in the apparent dNTP binding affinity of the insertion mutants is consistent with their kinetic parameters, we analyzed the steady-state kinetic parameters of these mutants.


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