Words similar to dmem

Example sentences for: dmem

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N2a mouse neuroblastoma cells stably transfected with the Swedish mutant form of APP (SweAPP N2a cells; APP695, 595–596 KM/NL) (gift from G. Thinakaran and S. Sisodia, University of Chicago, Chicago, Illinois, United States) were maintained in DMEM, 10% FBS, and 200 μg/ml G418 in the presence of penicillin and streptomycin [32].
Elutriation fractions with more than 50% and less than 75% monocytes were collected and concentrated by centrifugation at 1200 rpm for 5 min, and the cell pellets were combined, reconstituted in DMEM plus 20% sterile heat-inactivated FCS, counted, washed in medium, repelleted, and then resuspended in DMEM to 5 × 10 6/ml and dispensed into either tissue-culture plastic slides or glass chamber slides.
After centrifugation, the cultures were washed once with 0.5 ml DMEM, treated with 0.5 ml serum-free media containing 0.1 units/ml penicillin and 0.1 μg/ml streptomycin, and returned to the incubator.
Cultures were maintained with DMEM (R&D Systems) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin.
For fatty acid labeling, cells in a 6 cm dish were incubated with 1 ml of DMEM containing 10% dialyzed fetal bovine serum, 5 mM sodium pyruvate, and [9,10- 3H]palmitic acid (1 mCi/ml) or [9,10- 3H]myristate (0.