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Example sentences for: dmem

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Growth curve analysis of Infected populations and were pretreated for 5 days in phenol red-free, Dulbecco's modified eagle medium (DMEM)/F-12 medium (Gibco/Life Technologies) supplemented with 10% charcoal/dextran treated serum (Hyclone, Logan, UT, USA), then plated at 2.5 × 10 6cells per 100mm dish in triplicate in the absence or presence of estrogen (10 -8mol/l; 17β-Estradiol; 1,3,5 (10) - Estratriene 3,17β-diol; Sigma, St Louis, MO, USA).
In time-course studies, cells were treated with 100 nanomol/L 1,25-dihydroxyvitamin D diluted in DMEM + 5% FBS for 0, 2 h, 4 h, 8 h and 24 h.
RSV and TNFα were added to the DMEM/inhibitor mix to final concentrations as indicated in the figures.
Immortalized cells were maintained in Dulbecco's modified Eagle medium (DMEM, Sigma, St. Louis, MO) with 5% fetal bovine serum (FBS) and 5% calf serum in 5% CO 2 at 37°C.
Dulbecco's Modified Eagle Medium (DMEM), hypoxanthine-aminopterin-thymidine (HAT) medium supplement, foetal calf serum (FCS) and gentamycin were purchased from Gibco BRL (Rockville, Maryland).