Example sentences for: dmem

How can you use “dmem” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The wounded monolayers were incubated at 37°C in DMEM containing 10% FBS with or without mitomycin C (10 μg/ml, Sigma, St Louis, MO) to block mitosis.

  • Bovine aortic endothelial cells were cultured as previously described [ 23] using Dulbecco's modified Eagles medium (GIBCO) supplemented with 10% fetal bovine serum (Hyclone, Logan UT), 100 μg/ml streptomycin and 100 μg/ml penicillin (complete-DMEM).

  • Dermal cells were recovered by centrifugation of the digestion mixture at 5000 rpm in a table top centrifuge, and then cultured in 25 cm 2flasks in DMEM with 10% FBS and 1% penicillin-streptomycin.

  • For the O 2 - assay, rat neonatal microglia (250,000 cells/ 24-well cell culture clusters) were treated with (1) 1 mM domoic acid, (2) LPS-free water used as a vehicle for domoic acid or (3) 10 ng/ml LPS in a final volume of 1 ml of DMEM supplemented with 10% FBS + 120 U/ml P + 12 μg/ml S and incubated in a humidified 5% CO 2 incubator at 36°C for 1 to 6 hours.

  • The cells were then washed three times with DMEM without FBS and incubated with 3H-thymidine (1 μCi/ml; NEN, Boston, MA) in DMEM containing 10% FBS for time periods varying between 0 and 30 hours.


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