Example sentences for: dmem-hg

How can you use “dmem-hg” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Other media were phenol red-free DMEM-HG without estrogens (PhR-/E2-), or phenol red-free DMEM-HG with 20 nM E2 (PhR-/E2+).

  • Cells were seeded in 60 mm Falcon Primaria petris (Fisher Scientific, Pittsburgh, PA), in the amount of 7 × 10 6per dish cells in 3 ml DMEM-HG with 5% FBS + antibiotics as above, and cultured in an humidified atmosphere with 5% CO 2 at 37°C.

  • Trophoblast cultures from six additional term placentae were studied for trophoblast differentiation in normal DMEM-HG medium with phenol red (PhR+), in phenol red-free medium without E2 (PhR-/E2-), and in phenol red-free medium with 20 nM E2 (PhR-/E2+).

  • Placental tissue was digested and trophoblast cells isolated and cultured as described previously [ 15 36 ] . Briefly, the cells collected after digestion were pooled, washed twice in DMEM containing 25 mM HEPES and 4500 mg/L glucose (DMEM-HG), diluted to 10 ml in DMEM-HG supplemented with heat inactivated 5% fetal bovine serum (FBS) and antibiotics (50 μg/ml gentamycin, 100 U/ml penicillin, and 100 μg/ml streptomycin), and viable large nucleated cells (95% in trypan blue exclusion test) were counted.

  • Cells were grown in complete DMEM-HG, which contains 45 μM phenol red (PhR+).

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