Words similar to dimethylthiazol-
Example sentences for: dimethylthiazol-
How can you use “dimethylthiazol-” in a sentence? Here are some example sentences to help you improve your vocabulary:
15d-PGJ 2 , 15-deoxy-delta 12, 14-Prostaglandin J 2 ; ERK, Extracellular signal-Regulated Kinase; 4-HNE, 4-hydroxynonenal, MAP kinases, Mitogen-Activated Protein kinases; MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide); PBS, phosphate-buffered saline; PPARs, peroxisome proliferator-activated receptors; RPE, retinal pigment epithelium.
Cells were plated in 96-well plates (human primary RPE: 10,000 cells/well, ARPE-19: 15,000-20,000 cells/well, unless otherwise stated) overnight, fed with serum-free medium or treated with testing agents (prepared in serum-free medium) the next day followed by H 2 O 2 treatment for a day, then the viability from each treatment was determined by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay [ 27 ] . Culture medium was removed after treatment and then 100 μl MTT solution (100 μg/ml prepared in DMEM) was added to each well.
The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay of cytotoxic activity
ADR = adriamycin; DRO = droloxifen; GSTπ = glutathione S-transferase Pi; MDR = multidrug resistance; MG = megestrol; MRP = MDR-related protein; MTT = 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2 H -tetrazolium bromide; NOM = nomegestrol; PBS = phosphate-buffered saline; P-gp = P-glycoprotein; RT-PCR = reverse transcriptase polymerase chain reaction; TAM = tamoxifen; TopoIIα = topoisomerase IIα; VRP = verapamil.
The assay is based upon the cleavage of the yellow tetrazolium salt MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] to purple formazan crystals by metabolically active cells [ 15 ] . The tested concentration of Gleevec ranged from 0.5 to 8 μM.
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