Words similar to digoxigenin

Example sentences for: digoxigenin

How can you use “digoxigenin” in a sentence? Here are some example sentences to help you improve your vocabulary:

The in vitro transcription was performed for 2 hours at 37°C in reactions containing 100 ng of template DNA, 40 U of T7 RNA polymerase (for antisense probes) or 40 U of SP6 RNA polymerase (for sense probes), 40 mM Tris-HCl (pH 8.0), 6 mM MgCl 2 , 10 mM dithioerythritol, 2 mM spermidine, 10 mM NaCl, 1 mM each of ATP, GTP, and CTP, 0.65 mM UTP, 0.35 mM digoxigenin (DIG)-labeled UTP (or fluorescein-12-UTP), and 20 U RNasin (Promega, Madison, WI).
BAC and PAC DNAs, isolated from bacterial cultures (Nucleobond, Clontech, Franklin Lakes, NJ), were labeled by nick-translation with biotin and digoxigenin.
of maleate buffer with 0.5% blocking reagent to which was added anti digoxigenin Fab fragment antibody (diluted 20,000:1) conjugated with alkaline phosphatase (Boehringer Mannheim, Biochemical Products, Indianapolis IN).
All digoxigenin labeled probes for non-radioactive detection were generated using the Polymerase Chain Reaction.
The probe utilized was a 300 base pair digoxigenin labeled PCR product.