Example sentences for: digoxigenin

How can you use “digoxigenin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The digoxigenin-labeled RNA probe was quantified by immunological detection using a modified digoxigenin quantification protocol (Roche).

  • Hybridization was conducted at high stringency with a 1700 base pair homologous cDNA probe labeled with digoxigenin.

  • of maleate buffer with 0.5% blocking reagent to which was added anti digoxigenin Fab fragment antibody (diluted 20,000:1) conjugated with alkaline phosphatase (Boehringer Mannheim, Biochemical Products, Indianapolis IN).

  • The digoxigenin labeled probe was generated as described using the transcript 4 cycling parameters and primers T-41 and T-42.

  • The in vitro transcription was performed for 2 hours at 37°C in reactions containing 100 ng of template DNA, 40 U of T7 RNA polymerase (for antisense probes) or 40 U of SP6 RNA polymerase (for sense probes), 40 mM Tris-HCl (pH 8.0), 6 mM MgCl 2 , 10 mM dithioerythritol, 2 mM spermidine, 10 mM NaCl, 1 mM each of ATP, GTP, and CTP, 0.65 mM UTP, 0.35 mM digoxigenin (DIG)-labeled UTP (or fluorescein-12-UTP), and 20 U RNasin (Promega, Madison, WI).

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