Example sentences for: digoxigenin

How can you use “digoxigenin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The transcript 4 amplification product was used as template for the generation of a digoxigenin labeled probe for use in the subunit A transcript stress response experiment.

  • BAC and PAC DNAs, isolated from bacterial cultures (Nucleobond, Clontech, Franklin Lakes, NJ), were labeled by nick-translation with biotin and digoxigenin.

  • Hybridization was conducted at high stringency with a 1700 base pair homologous cDNA probe labeled with digoxigenin.

  • The in vitro transcription was performed for 2 hours at 37°C in reactions containing 100 ng of template DNA, 40 U of T7 RNA polymerase (for antisense probes) or 40 U of SP6 RNA polymerase (for sense probes), 40 mM Tris-HCl (pH 8.0), 6 mM MgCl 2 , 10 mM dithioerythritol, 2 mM spermidine, 10 mM NaCl, 1 mM each of ATP, GTP, and CTP, 0.65 mM UTP, 0.35 mM digoxigenin (DIG)-labeled UTP (or fluorescein-12-UTP), and 20 U RNasin (Promega, Madison, WI).

  • The probe utilized was a 300 base pair digoxigenin labeled PCR product.

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