Words similar to digoxigenin-
Example sentences for: digoxigenin-
How can you use “digoxigenin-” in a sentence? Here are some example sentences to help you improve your vocabulary:
[ 11 ] . Antisense and sense RNA probes generated from PCR of a 320-bp fragment (nt 1524-1843) from the last exon including the 3' UTR of human SPAM1 were labeled with digoxigenin-11-UTP (DIG) using an in vitro transcription system [Riboprobe ®System, (Promega, Cat.
BAC DNA (1 μg) was labeled with biotin-16-dUTP (Roche) or digoxigenin-11-dUTP (Roche) by nick translation.
We used human insulin cDNA (320 bp) cloned in plasmid pCR4-TOPO (Invitrogen) as a template for in vitro transcription to produce riboprobes with a digoxigenin-RNA labeling kit (Ambion, Austin, Texas, United States), performed hybridization with IPC cluster sections as described [16], and used DIG Nucleic Acid Detection Kit (Roche, Indianapolis, Indiana, United States) for development according to the manufacturer's instructions.
The two gene fragments were labelled separately with digoxigenin-11-dUTP (Boehringer Mannheim, Germany) by nick-translation (Life Technologies Inc., Gaithersburg, MD).
End-labeling of fragmented DNA was used to monitor progressive (nuclear) apoptosis [ 18 19 ] . Briefly, 3'-OH ends of DNA were linked with digoxigenin-11-d uridine triphosphate by terminal deoxynucleotidyl transferase (TdT) catalysis.