Example sentences for: digoxigenin-

How can you use “digoxigenin-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • [ 11 ] . Antisense and sense RNA probes generated from PCR of a 320-bp fragment (nt 1524-1843) from the last exon including the 3' UTR of human SPAM1 were labeled with digoxigenin-11-UTP (DIG) using an in vitro transcription system [Riboprobe ®System, (Promega, Cat.

  • The two gene fragments were labelled separately with digoxigenin-11-dUTP (Boehringer Mannheim, Germany) by nick-translation (Life Technologies Inc., Gaithersburg, MD).

  • The purified PCR product (5 μl) was transcribed in 96-well format by incubation for 2 h at 37°C after the addition of 5 μl of a polymerase reaction cocktail consisting of 2 U T7 RNA polymerase, 4.6 U RNase inhibitor, 10 mM NTPs, 3.5 mM digoxigenin-11-UTP, 40 mM Tris pH 8.0, 6 mM MgCl 2 , 10 mM DTT and 2 mM spermidine.

  • End-labeling of fragmented DNA was used to monitor progressive (nuclear) apoptosis [ 18 19 ] . Briefly, 3'-OH ends of DNA were linked with digoxigenin-11-d uridine triphosphate by terminal deoxynucleotidyl transferase (TdT) catalysis.

  • We used human insulin cDNA (320 bp) cloned in plasmid pCR4-TOPO (Invitrogen) as a template for in vitro transcription to produce riboprobes with a digoxigenin-RNA labeling kit (Ambion, Austin, Texas, United States), performed hybridization with IPC cluster sections as described [16], and used DIG Nucleic Acid Detection Kit (Roche, Indianapolis, Indiana, United States) for development according to the manufacturer's instructions.

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