Example sentences for: digoxigenin-

How can you use “digoxigenin-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The purified PCR product (5 μl) was transcribed in 96-well format by incubation for 2 h at 37°C after the addition of 5 μl of a polymerase reaction cocktail consisting of 2 U T7 RNA polymerase, 4.6 U RNase inhibitor, 10 mM NTPs, 3.5 mM digoxigenin-11-UTP, 40 mM Tris pH 8.0, 6 mM MgCl 2 , 10 mM DTT and 2 mM spermidine.

  • The two gene fragments were labelled separately with digoxigenin-11-dUTP (Boehringer Mannheim, Germany) by nick-translation (Life Technologies Inc., Gaithersburg, MD).

  • [ 11 ] . Antisense and sense RNA probes generated from PCR of a 320-bp fragment (nt 1524-1843) from the last exon including the 3' UTR of human SPAM1 were labeled with digoxigenin-11-UTP (DIG) using an in vitro transcription system [Riboprobe ®System, (Promega, Cat.

  • BAC DNA (1 μg) was labeled with biotin-16-dUTP (Roche) or digoxigenin-11-dUTP (Roche) by nick translation.

  • We used human insulin cDNA (320 bp) cloned in plasmid pCR4-TOPO (Invitrogen) as a template for in vitro transcription to produce riboprobes with a digoxigenin-RNA labeling kit (Ambion, Austin, Texas, United States), performed hybridization with IPC cluster sections as described [16], and used DIG Nucleic Acid Detection Kit (Roche, Indianapolis, Indiana, United States) for development according to the manufacturer's instructions.


How many words do you know? Try our free vocabulary size test!


Search

Search for example sentences

Loading Loading...
Quantcast