Example sentences for: difco

How can you use “difco” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Mycobacterium smegmatis LR222 strains were grown in Middlebrook 7H9 broth supplemented with 10% albumin-dextrose-catalase (ADC, v/v, Difco Laboratories, Detroit, MI) and 0.05% Tween 80 (v/v, Sigma, St. Louis, MO) at 37°C, with agitation on a rotating platform (100 rpm), or were grown on trypticase soy agar (TSA) plates.

  • 0 mL of sterile 100 mM phosphate buffer (pH 7.5), 2 mL of 100X Nutrient Broth as a biological reductant (Difco), and 1.0 mL of 1 mM DCIP to a sterile 25 mL serum bottle.

  • A small amount of water (1 ml) was added and 0.1 ml of the suspension was quickly removed using an aseptic technique and spread onto a plate of nutrient agar (Difco).

  • To quantitate biofilm formation, 20 μl of Difco crystal violet (Becton Dickinson, Sparks, Maryland) was added to each well and incubated at room temperature for 15 minutes.

  • Chicken type II collagen was used for generation of arthritis as described elsewhere [ 26 ] . Male and female WT, heterozygous IL-10 +/-and IL-10 -/-mice were immunized once with 100 μg chicken type II collagen emulsified in complete Freund's adjuvant (CFA) (Difco, Detroit, MI, USA) by intradermal injection at the base of the tail.


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