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Example sentences for: difco

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The bacteria used in this study were the opacity factor positive M49 Streptococcus pyogenes isolate CS101 and an isogenic β-hemolytic negative variant generated by transposon mutagenesis, CS101 sagA/pel ::Tn 917 [ 4 ] . Todd-Hewitt broth containing 0.3% yeast extract (THY) was obtained from DIFCO (Detroit MI).
Reagents for bacterial and yeast culture were purchased from Difco.
Four types of media containing 2% granulated agar (Difco, Detroit, MI) were used to grow fungi: (1) potato dextrose agar [ 31 ] , (2) potato sucrose agar (similar to potato dextrose agar except sucrose is used instead of dextrose; [ 31 ] , (3) casein agar (for 1 L, 5 g casein, 0.5 g NaCl, 20 g sucrose, and 20 g agar), and (4) sagebrush-leaf agar.
To quantitate biofilm formation, 20 μl of Difco crystal violet (Becton Dickinson, Sparks, Maryland) was added to each well and incubated at room temperature for 15 minutes.
injections of 100 μl containing a mixture of 200 μg each of the G7 peptides, the PD peptides or the HEL/OVA peptides, dissolved in 50 μl of sterile, pyrogen-free 0.9% NaCl ("saline") and emulsified in an equal volume of incomplete Freund's adjuvant (IFA) (Difco Laboratories, Detroit, MI).