Example sentences for: difco

How can you use “difco” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The bacteria used in this study were the opacity factor positive M49 Streptococcus pyogenes isolate CS101 and an isogenic β-hemolytic negative variant generated by transposon mutagenesis, CS101 sagA/pel ::Tn 917 [ 4 ] . Todd-Hewitt broth containing 0.3% yeast extract (THY) was obtained from DIFCO (Detroit MI).

  • Reagents for bacterial and yeast culture were purchased from Difco.

  • Four types of media containing 2% granulated agar (Difco, Detroit, MI) were used to grow fungi: (1) potato dextrose agar [ 31 ] , (2) potato sucrose agar (similar to potato dextrose agar except sucrose is used instead of dextrose; [ 31 ] , (3) casein agar (for 1 L, 5 g casein, 0.5 g NaCl, 20 g sucrose, and 20 g agar), and (4) sagebrush-leaf agar.

  • To quantitate biofilm formation, 20 μl of Difco crystal violet (Becton Dickinson, Sparks, Maryland) was added to each well and incubated at room temperature for 15 minutes.

  • injections of 100 μl containing a mixture of 200 μg each of the G7 peptides, the PD peptides or the HEL/OVA peptides, dissolved in 50 μl of sterile, pyrogen-free 0.9% NaCl ("saline") and emulsified in an equal volume of incomplete Freund's adjuvant (IFA) (Difco Laboratories, Detroit, MI).

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