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Example sentences for: difco

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The E. coli (pYUB178::H37Rv) library was grown in Luria broth (LB) (Difco Laboratories, Detroit, Mich.) containing 50 μg kanamycin/mL (Sigma Chemical Company, St. Louis, Mo.)
Growth medium components and agar were from Difco.
Four types of media containing 2% granulated agar (Difco, Detroit, MI) were used to grow fungi: (1) potato dextrose agar [ 31 ] , (2) potato sucrose agar (similar to potato dextrose agar except sucrose is used instead of dextrose; [ 31 ] , (3) casein agar (for 1 L, 5 g casein, 0.5 g NaCl, 20 g sucrose, and 20 g agar), and (4) sagebrush-leaf agar.
injections of 100 μl containing a mixture of 200 μg each of the G7 peptides, the PD peptides or the HEL/OVA peptides, dissolved in 50 μl of sterile, pyrogen-free 0.9% NaCl ("saline") and emulsified in an equal volume of incomplete Freund's adjuvant (IFA) (Difco Laboratories, Detroit, MI).
To quantitate biofilm formation, 20 μl of Difco crystal violet (Becton Dickinson, Sparks, Maryland) was added to each well and incubated at room temperature for 15 minutes.