Example sentences for: dids

How can you use “dids” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Membrane potential was further depolarized in GC exposed to 4-AP in the presence of DIDS (Figure 8B), consistent with the hypothesis that 4-AP-induced hyperpolarization depends on chloride influx.

  • The observed effects of Cl -channel antagonists differ significantly between the present study where DIDS prevented 4-AP from decreasing progesterone synthesis and previous investigations where DIDS and other Cl -channel antagonists prevented LH from enhancing progesterone production [ 29 35 56 ] . However, this discrepancy is expected, because DIDS antagonizes a chloride influx pathway in pig GC, and chloride efflux pathways in Leydig cells.

  • Interestingly, concomitant exposure to DIDS prevented 4-AP from diminishing the accumulation of progesterone in GC culture media (Figure 8C), suggesting that chloride influx, membrane hyperpolarization or both might mediate this effect of 4-AP.

  • To test this hypothesis, the effects of 4-AP on membrane potential were examined using GC incubated in either media modified to contain the chloride channel antagonist DIDS, or a physiological buffer solution modified to contain a low (8 mM) concentration of chloride.

  • 4-aminopyridine (4-AP); analysis of variance (ANOVA); 8-(4-chlorophenylthio)adenosine-3',5'-cyclic monophosphorothioate (8-CPT-cAMP); 4',4' diisothicyanato-stilbene-2-2'-disulfonic acid (DIDS); bis-(1,3-dibutylbarbituric acid)trimethine oxonol (DiBAC 4 (3)); 5α-dihydrotestosterone (5α-DHT); Dulbecco's modified eagle's medium (DMEM); enhanced chemiluminescence (ECL); ethylenediaminetetraacetic acid (EDTA); fetal bovine serum (FBS); follicle stimulating hormone (FSH); granulosa cells (GC); human chorionic gonadotropin (hCG); 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES); 3β-hydroxysteroid dehydrogenase (3β-HSD); 3-isobutyl-1-methylxanthine (IBMX); least significant difference (LSD); luteinizing hormone (LH); mitogen activated protein kinase (MAPK); 1,3-Benzenedicarboxylic acid, 4,4'-[1,4,10,13-tetraoxa-7,16-diazacyclooctadecane-7,16-diylbis(5-methoxy-6,2-benzofurandiyl)]bis-, tetrakis [(acetyloxy)methyl] ester (PBFI-AM); phosphate-buffered saline (PBS); proliferating cell nuclear antigen (PCNA); protein kinase A (PKA); sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE); steroidogenic acute regulatory protein (StAR); tris-buffered saline (TBS).


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