Words similar to dap

Example sentences for: dap

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Enhanced specificity against mismatches has also been observed by other groups when DAP is incorporated into PNA oligomers [ 13 ] and after C5-(1-propynyl)ation of pyrimidines in DNA-RNA duplexes [ 33 ] .
Many studies have shown that a ribopurine rich strand bound to a deoxypyrimidine rich strand has a higher stability than the corresponding all deoxy strands which, in turn, have a higher stability than a deoxypurine rich strand bound to a ribopyrimidine rich strand [ 26 27 ] . These differences also suggest that further studies examining DAP or MeC incorporation into first strand cDNA, followed by formation of a DNA-DNA duplex, may not necessarily show the same effects as incorporation of these modified nucleotides into cRNA.
Remarkably, in one sequence context, the DAP modification enhanced specificity against mismatches.
Figure 6Bshows that the 1:1 DAP:A condition produces ratios with similar overall variability (average CV of 12.
Recently, studies employing DNA arrays have also examined the use of modified nucleotides such as DAP, 5-bromodeoxyuridine, and 2'-O-methylthymidine in the probe [ 21 22 ] . An alternative method to increase the probe-target affinity is to incorporate the modified ribonucleotide, as the triphosphate derivative, during the IVT process so that the cRNA produced has the desired level of substitution of the corresponding unmodified nucleotide.