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Example sentences for: daoy

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This reporter plasmid contains three optimal TCF binding sites [ 28 ] . TOPFLASH luciferase activity was activated 41-fold by S37F mutant β-catenin and 3.8 fold by wild-type β-catenin in DAOY cells (Fig.
First, co-transfection of the cDNA used to make our transgenic mice and the TOPFLASH Wnt pathway reporter plasmid into DAOY medulloblastoma cells resulted in over forty-fold induction of reporter activity.
In order to evaluate the transcriptional activity of FLAG epitope-tagged β-catenin in a medulloblastoma cell line, the S37F and wild-type constructs were introduced into DAOY cells along with the Wnt pathway TOPFLASH reporter.
Medulloblastoma DAOY cells were obtained from the American Type Culture Collection and maintained in Improved MEM Zn ++Option (Richter's Modification) medium containing supplemented with 20% fetal bovine serum (Life Technologies, Inc., USA).
Transcriptional activity of β-catenin constructs in medulloblastoma DAOY cells