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Example sentences for: daoy

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Transcriptional activity of β-catenin constructs in medulloblastoma DAOY cells
This reporter plasmid contains three optimal TCF binding sites [ 28 ] . TOPFLASH luciferase activity was activated 41-fold by S37F mutant β-catenin and 3.8 fold by wild-type β-catenin in DAOY cells (Fig.
Medulloblastoma DAOY cells were obtained from the American Type Culture Collection and maintained in Improved MEM Zn ++Option (Richter's Modification) medium containing supplemented with 20% fetal bovine serum (Life Technologies, Inc., USA).
In order to evaluate the transcriptional activity of FLAG epitope-tagged β-catenin in a medulloblastoma cell line, the S37F and wild-type constructs were introduced into DAOY cells along with the Wnt pathway TOPFLASH reporter.
First, co-transfection of the cDNA used to make our transgenic mice and the TOPFLASH Wnt pathway reporter plasmid into DAOY medulloblastoma cells resulted in over forty-fold induction of reporter activity.