Words similar to crosslinking
Example sentences for: crosslinking
How can you use “crosslinking” in a sentence? Here are some example sentences to help you improve your vocabulary:
Btk kinase activity and tyrosine phosphorylation increase upon stimulation of sIgM as well as the IL-5 and IL6 receptors in B cells, and upon crosslinking of the high affinity IgE receptor (FcεRII) in mast cells [ 12, 13, 14, 15].
Although extraction can be done before, during, or after fixation, we used simultaneous extraction and fixation, because (i) cytoskeletal molecules may be rearranged or altered during fixation, as irreversible crosslinking of polypeptides is not complete even after 15 min incubation with 4% PFA at 37°C; (ii) although actin was more difficult to extract, moesin and other polypeptides were removed already with 0.5% DOTMAC in the absence of PFA.
We used Lymphochip microarrays to profile the changes in gene expression that occurred in response to a wide variety of signaling events: B-cell lymphoma cell lines stimulated by crosslinking the B-cell antigen receptor or by treatment with phorbol ester and ionomycin (PI), resting peripheral blood T cells stimulated with PI, and peripheral blood monocytes stimulated with lipopolysaccharide.
Because our integrin-CD98 complex was observed in light membrane fractions and not in dense sucrose gradient fractions, and because we could not capture the complex by covalent crosslinking (not shown), the interaction may not be direct.
Fractionation by SDS-polyacrylamide gel electrophoresis and autoradiography of the resulting immunocomplexes from these cells showed crosslinking exclusively to HA-Smo, but with an efficiency of less than 1% (data not shown).
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