Words similar to crosslinked

Example sentences for: crosslinked

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Only a small amount of polypeptides was extracted when cells were fixed with glutaraldehyde or DSP-Tsb (Figure 6B, lane 2, 3 and 6) and the cells were morphologically well preserved by these fixatives as observed by SEM, indicating that most proteins were irreversibly crosslinked.
DNA was crosslinked to the membrane by exposure to 1200 mjoules of ultraviolet light in a UV-STRATALINKER 1800 (STRATAGENE, LaJolla, CA).
On the other hand, when cells were fixed with PLP or LP and treated with 1% saponin in 3% BSA for permeabilization, most of the polypeptides were either not crosslinked by these relatively mild fixatives or crosslinks were reversed by the addition of SDS sample buffer (Figure 6B, lanes 4 and 5).
At 37°C, the required time for incubation can be shortened, but most of the actin molecules were not irreversibly crosslinked even after 30 min.
Under these conditions, moesin and β-actin migrated predominantly as monomeric polypeptides at 45 and 78 kDa positions on the SDS-PAGE gels and only very small amounts of crosslinked moesin and β-actin were detected by western blotting at higher molecular weight positions (data not shown).