Example sentences for: cloning

How can you use “cloning” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • We used genomic DNA for a template, and for each mutant transcribed the complete ftsZ sequence (adding NdeI and BamHI cloning sites at the ends) using error-correcting Pfu polymerase [ 37] and outward-facing primers containing the desired mutations.

  • hprt replacement vector contains a 5' arm of hprt homology consisting of 3.4 kb of mouse 5' hprt genomic DNA flanking a unique Not1 cloning site.

  • Mutazyme (Stratagene, La Jolla, CA) was used to amplify PcrG for cloning into pBAD18 [ 33 ] . The ligation reaction was electroporated into lcrG Y. pestis (which is temperature sensitive due to its Ca 2+-blind phenotype) and the cells were plated on TMH medium [ 29 ] containing arabinose, Ca 2+, kanamycin and carbenicillin.

  • The success of the cloning procedure was initially monitored by picking 5 clones from each group, preparing plasmid DNA and sequencing.

  • The T gene was cloned in 1990 [ 10 ] , and its expression pattern was found to correlate with the tissue types affected in T mutants [ 8 ] . Subsequently, T protein was shown to bind specifically to DNA and its preferred in vitro target sequence was identified [ 11 ] . It was further shown that T encodes a transcription factor capable of regulating expression of a reporter via the identified target sequence [ 12 ] . In their report describing the initial cloning of the T gene, Herrmann et al.


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