Words similar to cloned

Example sentences for: cloned

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After polishing the ends of the fragments using Klenow (New England Biolabs, Beverly, MA), they were cloned into SmaI -restricted/CIAP pUC18 vector.
After sequence confirmation, the ATF-BPTI cDNA was cloned into the recombinant adenovirus (Ad) shuttle vector Adapt.
Following SSH, subtracted products were cloned and 96 template clones for each subtracted product of each comparison were randomly selected and differentially screened.
Myosin II heavy chain kinase (MHCK) activity in this system capable of disassembling myosin II filaments in vitro was originally reported with partially enriched kinase fractions [ 13 ] . The enzyme MHCK-A was subsequently purified to homogeneity and shown to be capable of driving myosin II filament disassembly in vitro via myosin II heavy chain phosphorylation [ 14 15 ] ). A MHCK-A cDNA was cloned via expression cloning and peptide sequence derived from the native enzyme [ 16 ] . This enzyme is now recognized as the founding member of a highly novel family of protein kinases unrelated to conventional protein kinases, with members present in D. discoideum and throughout the animal kingdom.
The lamin B-SKP2 cassette was removed and cloned into the pADTRACK1 shuttle vector.