Words similar to cloned

Example sentences for: cloned

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cDNA representing the rat GnRH receptor was cloned into pcDNA3 as described and validated previously [ 22 23 ] . cDNAs representing RGS3 and RGS3T (RGS3 amino acids 314-519) were cloned into the eukaryotic expression vector, rcCMV, as described [ 24 ] . COS-1 cells cultured as detailed before [ 23 ] were co-transfected with GnRH receptor cDNA and RGS3 or RGS3T cDNA using Lipofectamine (Life Technologies, Gaithersburg, MD) [ 23 ] , and 72 h later, treated with 10 -7M GnRH-A for 0, 10, 20, or 30 sec before being collected for IP 3 measurements [ 22 23 ] .
After the first aquaporin from plants (γ-TIP) was cloned and functionally expressed [ 29], plant AQPs received considerable attention.
The complete SKP2 cDNA or a mutant lacking the F-box (SKP2-ΔF) were cloned into a modified pcDNA3 plasmid providing an N-terminal Myc epitope tag and the human lamin B leader sequence.
JYJ cloned, expressed, and performed initial characterization studies of recombinant Ppt1p, performed gene replacement, immunoblot and immunolocalization studies, and prepared the manuscript.
Mutations were introduced into full-length wild-type and mutant EGFR cDNAs using a QuikChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, California, United States) and cloned into expression vectors as described [3].