Words similar to cloned

Example sentences for: cloned

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Inserts were directionally cloned between an Eco RI site (5') and a Xho I site (3'); however, sequencing indicates that ~22% of clones are in reverse orientation.
Since the bacterial genes are typically not cloned at a genome-wide scale as non-redundant, ordered sets, as with eukaryotic cDNA libraries, it is not possible to use 'universal primers' (e.g.
In order to examine the clonal variation within other Cpn 1054 family members, sequences surrounding the polyC tracts were amplified from selected genes and the products cloned into plasmids.
Short cytoplasmic domains (5-14 amino acids) are found at both the amino and carboxyl termini of CD9 [ 14 ] . As reported previously, we used a yeast two-hybrid system and observed interaction between the second extracellular domain of MkCD9 and the extracellular domain of proHB-EGF [ 9 ] . However, despite repeated attempts, using the yeast two-hybrid system, we were unable to show interaction between the amino terminal cytoplasmic domain of monkey CD9 and the cytoplasmic domain of proHB-EGF; these results may reflect the limitation of the yeast two-hybrid system as the insert sizes cloned into the vectors may be too small resulting in Gal4 fusion proteins whose "test subunits" are masked by the Gal4 subunit.
The cloned receptor is called VR1 (vanilloid receptor subtype 1) [ 9 10 ] . The nucleotide sequence of VR1 predicts a protein of 838 amino acids with a molecular mass of 95 kDa.