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Example sentences for: clontech

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Templates included total human RNA from placenta, kidney, brain, thymus or uterus (Clontech).
Using an Advantage-GC cDNA PCR polymerase (Clontech) and Pfu DNA polymerase (Stratagene), PCR was carried out with primers specific to the exon and/or intron of DMN sequence (Table 1) under the following conditions: 94°C for 1 min followed by 35 cycles of 94°C for 1 min, 57-59 °C for 1 min, 72°C for 3 min and a final extension time of 10 min at 72°C.
To extend the known 5' portion of the gene's coding sequence, an adult human kidney library (Clontech, cat.
The triple auxotrophic transformants were tested for β-galactosidase activity using the filter paper assay described in the manual provided by Clontech.
The plasmid, pVAX-LacZ, (Clontech, Palo Alto, CA, USA) was used as transfection control.