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Example sentences for: clontech

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Mouse Smo and Ptc1 genes were introduced by a retroviral approach utilizing the pLPCX vector (Clontech; Palo Alto, USA) to limit the copy number per cell.
Application-targeted arrays and array reagents are already commercially available (Operon, Clontech, Incyte Pharmaceuticals, Affymetrix) for research in diverse areas such as cancer, stress and aging, toxicology, hematology, cell cycle, neurology and apoptosis.
Human umbilical vein endothelial cell (EC) cultures were purchased (Clontech, Palo Alto, CA) and maintained at 30-80% confluency to ensure high viability.
Using an Advantage-GC cDNA PCR polymerase (Clontech) and Pfu DNA polymerase (Stratagene), PCR was carried out with primers specific to the exon and/or intron of DMN sequence (Table 1) under the following conditions: 94°C for 1 min followed by 35 cycles of 94°C for 1 min, 57-59 °C for 1 min, 72°C for 3 min and a final extension time of 10 min at 72°C.
Cell line HG2551a was transfected [ 10] with normal and mutant GFP-BLM genes cloned behind the Tet-responsive promoter in plasmid pTRE (Clontech).