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Example sentences for: clontech

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These include: the glass-slide based oligonucleotide array system [ 2, 3, 4] developed by Affymetrix; the glass-slide-based cDNA clone Gene Expression Microarray (GEM) line from Incyte Genomics (IGI); the filter and glass-slide-based 'Atlas' arrays developed by Clontech Laboratories, composed of selected regions of PCR-amplified gene sequences; filter-based GeneFilters from Research Genetics (RGI) which, like IGI's GEMs, utilize partial or full-length cDNA clones.
To extend the known 5' portion of the gene's coding sequence, an adult human kidney library (Clontech, cat.
Positive control hybridization of a human β-actin cDNA probe (Clontech) to MTN 1 and 2 (Figure 2) confirmed the uniform loading of mRNA in each lane.
Hybridization was done in ExpressHyb solution (Clontech) at 60°C using a 32P dCTP-labeled probe that was synthesized from a 0.9 Kb template located in intron 5 of Pten [ 25] using the RTS RadPrime DNA labeling system (Life Technologies).
Total RNA was purified from PBMC samples using Clontech Nucleospin RNA II kit reagents (Clontech, Palo Alto, CA) and from whole blood samples using MRC TRI Reagent BD (Molecular Research Center, Cincinnati, OH, USA), an adaptation of the Chomczynski single-step method [ 16 ] , according to the manufacturer's instructions.