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Example sentences for: clontech

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The membrane was prehybridized for 0.5 h at 65°C, hybridized for 1 h at 65°C with random primer-labeled lac operator concatamer (40 copies) probe in ExpressHyb hybridization solution (Clontech Laboratories, CA), and then washed at 60°C in washing solution (0.
The PCR-Select cDNA Subtraction and PCR-Select Differential Screening Kits (Clontech Laboratories Inc.) were used to enrich for and identify differentially expressed genes in the driver and tester cDNAs [ 48 49 ] . Both forward- and reverse-subtraction were performed; untreated HUVEC cDNA served as driver and VEGF-treated HUVEC cDNA served as tester in the former, whereas untreated HUVEC cDNA served as tester and VEGF-treated HUVEC cDNA served as driver in the latter [ 48 49 ] . Target cDNA fragments were amplified using the Marathon™ cDNA Amplification Kit, subcloned into pCR ®2.
Yeast strain yVT87 (EGY48 (Clontech) MATa, ura3, his3, trp1, leu2::lexA6op- LEU2 ) was transformed by the method of Gietz and Schiestl (Agatep, R., R.D.
We examined the expression pattern of YL-1 using this RT-PCR product as a probe on a Clontech Human multiple tissue Northern blot (CAT# #7760-1).
Table 2shows the results of an analysis using Clontech's Human Cancer 1.2 K Array and total RNA from SW1353 cells treated with IL-1β (10 ng/ml) for 2 hours.