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Example sentences for: cl

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The microvasculature of the CL is thought to be the first structure to undergo degeneration via apoptosis during luteolysis [ 2 8 ] . In contrast to information available on steroidogenic cells of the CL, a gap exists in our understanding of the factors and their associated signaling pathways that initiate apoptosis in the vascular component of the CL.
However, the microvasculature of the CL is composed of a number of different luteal endothelial cell types; the most common of which does not express mRNA encoding the PGF
P. falciparum (3D7 or Dd2) was grown in human A-positive erythrocytes and infected cells were harvested at about 15% parasitemia and lysed by the saponin method essentially as described [ 14 15 ] . PfPP5 was partially purified using published procedures [ 18 ] . In brief, the parasites were resuspended in buffer A (50 mM Tris-Cl, pH 7.5, 10% glycerol, 1 mM DTT) plus 20 mM NaCl, 1% Triton X-100, leupeptin (1 μg/ml), and aprotinin (1 μg/ml) at an approximate protein concentration of 10 mg per ml.
The cell pellet was washed once in cold phosphate-buffered saline and subsequently resuspended in hypotonic lysis buffer (10 mM Tris.Cl, pH 7.9, 10 mM KCl, 1.5 mM MgCl2, 1 mM phenylmethylsulfonyl fluoride, 2 μg/ml pepstatin, 2 μg/ml leupeptin, 5 μg/ml aprotinin, 1 mM dithiothreitol).
During the life of the CL, low to moderate expression was seen in the lutein, but not the endothelial cells (Fig.