Words similar to cla

Example sentences for: cla

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The gel-purified ~400-bp fragment was inserted into the Cla I and Hin cII sites of p3002PCR (the plasmid containing core ars3002 generated by PCR; [ 10]).
The resulting plasmid was then cut with Eco RI, blunt-ended with Klenow polymerase and dNTPs, and self-religated, in order to destroy the Eco RI site at the boundary between core ars3002 and the enhancer that was regenerated during the process of Cla I linker addition.
In this vector, the TATA box and the initiation transcription site in URA3 are separated by Xho I- Cla I sites, which were used to clone targeting hooks.
The second pair contained 56-bp and 60-bp specific sequences from the 5' and 3' ends of human HPRT . The hooks were amplified from HPRT yeast artificial chromosome (YAC) [ 6 ] with primer pairs HP-ARS-F and HP-ARS-R (Table 2) and cloned into the Xho I- Cla I sites of pARS-NK, yielding the vector pARS-HPRT.
To evaluate these deletions in the context of extended ars3002 , the ~400-bp Cla I- Hin cII fragment containing the replication enhancer (from pUra4script: ars3002 (ext)) was inserted between the Cla I and Hin cII sites of the deletion constructs Δ1-Δ14 previously described [ 10].