Words similar to cla

Example sentences for: cla

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The second pair contained 56-bp and 60-bp specific sequences from the 5' and 3' ends of human HPRT . The hooks were amplified from HPRT yeast artificial chromosome (YAC) [ 6 ] with primer pairs HP-ARS-F and HP-ARS-R (Table 2) and cloned into the Xho I- Cla I sites of pARS-NK, yielding the vector pARS-HPRT.
To evaluate these deletions in the context of extended ars3002 , the ~400-bp Cla I- Hin cII fragment containing the replication enhancer (from pUra4script: ars3002 (ext)) was inserted between the Cla I and Hin cII sites of the deletion constructs Δ1-Δ14 previously described [ 10].
The first pair amplifies the ADH1 promoter as an Apa I- Xho I fragment; the second amplifies the URA3 gene as a Cla I- Bam HI fragment.
The hooks were PCR-amplified from the SV800 vector using specific primer pairs SV-ARS-F and SV-ARS-R (Table 2) and cloned into Xho I- Cla I sites of pARS-VN, yielding the vector pARS-TGAC.
The resulting plasmid was then cut with Eco RI, blunt-ended with Klenow polymerase and dNTPs, and self-religated, in order to destroy the Eco RI site at the boundary between core ars3002 and the enhancer that was regenerated during the process of Cla I linker addition.