Words similar to cla

Example sentences for: cla

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The gel-purified fragment was ligated with Cla I linker and then cut with Cla I and Hin cII to produce the ~400-bp fragment containing the enhancer [ 7].
To determine the distance permitted between the TATA box and the transcription initiation site in pARS-VN, we cloned different sized Alu targeting hooks (45/45 bp, 45/65 bp, 45/75 bp, 65/65 bp, 75/75 bp, and 115/75 bp) into vector Xho I- Cla I sites.
In this vector, the TATA box and the initiation transcription site in URA3 are separated by Xho I- Cla I sites, which were used to clone targeting hooks.
To evaluate these deletions in the context of extended ars3002 , the ~400-bp Cla I- Hin cII fragment containing the replication enhancer (from pUra4script: ars3002 (ext)) was inserted between the Cla I and Hin cII sites of the deletion constructs Δ1-Δ14 previously described [ 10].
The second pair contained 56-bp and 60-bp specific sequences from the 5' and 3' ends of human HPRT . The hooks were amplified from HPRT yeast artificial chromosome (YAC) [ 6 ] with primer pairs HP-ARS-F and HP-ARS-R (Table 2) and cloned into the Xho I- Cla I sites of pARS-NK, yielding the vector pARS-HPRT.