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Example sentences for: cla

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To evaluate these deletions in the context of extended ars3002 , the ~400-bp Cla I- Hin cII fragment containing the replication enhancer (from pUra4script: ars3002 (ext)) was inserted between the Cla I and Hin cII sites of the deletion constructs Δ1-Δ14 previously described [ 10].
The resulting plasmid was then cut with Eco RI, blunt-ended with Klenow polymerase and dNTPs, and self-religated, in order to destroy the Eco RI site at the boundary between core ars3002 and the enhancer that was regenerated during the process of Cla I linker addition.
The hooks were PCR-amplified from the SV800 vector using specific primer pairs SV-ARS-F and SV-ARS-R (Table 2) and cloned into Xho I- Cla I sites of pARS-VN, yielding the vector pARS-TGAC.
To determine the distance permitted between the TATA box and the transcription initiation site in pARS-VN, we cloned different sized Alu targeting hooks (45/45 bp, 45/65 bp, 45/75 bp, 65/65 bp, 75/75 bp, and 115/75 bp) into vector Xho I- Cla I sites.
In this vector, the TATA box and the initiation transcription site in URA3 are separated by Xho I- Cla I sites, which were used to clone targeting hooks.