Words similar to cla

Example sentences for: cla

How can you use “cla” in a sentence? Here are some example sentences to help you improve your vocabulary:

The hooks were PCR-amplified from the SV800 vector using specific primer pairs SV-ARS-F and SV-ARS-R (Table 2) and cloned into Xho I- Cla I sites of pARS-VN, yielding the vector pARS-TGAC.
The gel-purified fragment was ligated with Cla I linker and then cut with Cla I and Hin cII to produce the ~400-bp fragment containing the enhancer [ 7].
The resulting plasmid was then cut with Eco RI, blunt-ended with Klenow polymerase and dNTPs, and self-religated, in order to destroy the Eco RI site at the boundary between core ars3002 and the enhancer that was regenerated during the process of Cla I linker addition.
To evaluate these deletions in the context of extended ars3002 , the ~400-bp Cla I- Hin cII fragment containing the replication enhancer (from pUra4script: ars3002 (ext)) was inserted between the Cla I and Hin cII sites of the deletion constructs Δ1-Δ14 previously described [ 10].
In this vector, the TATA box and the initiation transcription site in URA3 are separated by Xho I- Cla I sites, which were used to clone targeting hooks.