Words similar to cla

Example sentences for: cla

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The second pair contained 56-bp and 60-bp specific sequences from the 5' and 3' ends of human HPRT . The hooks were amplified from HPRT yeast artificial chromosome (YAC) [ 6 ] with primer pairs HP-ARS-F and HP-ARS-R (Table 2) and cloned into the Xho I- Cla I sites of pARS-NK, yielding the vector pARS-HPRT.
To determine the distance permitted between the TATA box and the transcription initiation site in pARS-VN, we cloned different sized Alu targeting hooks (45/45 bp, 45/65 bp, 45/75 bp, 65/65 bp, 75/75 bp, and 115/75 bp) into vector Xho I- Cla I sites.
The resulting plasmid was then cut with Eco RI, blunt-ended with Klenow polymerase and dNTPs, and self-religated, in order to destroy the Eco RI site at the boundary between core ars3002 and the enhancer that was regenerated during the process of Cla I linker addition.
The first pair amplifies the ADH1 promoter as an Apa I- Xho I fragment; the second amplifies the URA3 gene as a Cla I- Bam HI fragment.
The gel-purified ~400-bp fragment was inserted into the Cla I and Hin cII sites of p3002PCR (the plasmid containing core ars3002 generated by PCR; [ 10]).