Example sentences for: chromatographic

How can you use “chromatographic” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To identify whether PfPP5 is actually expressed in the parasite, cell-free extracts of the asynchronous erythrocytic stage parasites were subjected to standard chromatographic procedures that had been previously optimized for human PP5 [ 18 ] . Studies of mammalian PP5 have revealed that the TPR domain might act as an auto-inhibitory domain of the phosphatase activity, and that tryptic digestion of PP5 causes loss of the TPRs and concomitant stimulation of activity [ 19 20 21 ] . As described under Materials and Methods, the purification was in fact monitored by assaying for a trypsin-activated phosphatase with the assumption that PfPP5 may behave like mammalian PP5 in this regard [ 18 20 21 ] . As mentioned, the activity peaks always coincided with a ~69 kDa band in parallel Western analyses of the chromatographic fractions.

  • Samples of 20 to 50 μL were injected onto a Discovery Amide C16 or Discovery C18 reverse phase column (15 cm × 4.6 mm, 5 μm, Supelco, Bellefonte, PA), and analytes were separated using the ion-pair chromatographic method.

  • All the chromatographic separations were carried out at 4°C.

  • The concentration and types of phytoestrogens in collected plasma samples were analyzed by gas-chromatography/mass spectrometry (GC/MS), as previously performed in our laboratories [ 28 40 51 ] . This was accomplished by liquid-solid extraction and liquid-gel chromatographic techniques to isolate the phytoestrogen fractions using standard assay methods with internal controls [ 28 ] .

  • Chromatographic peaks were integrated on an Everex 386/20 PC using Omega software and Perkin-Elmer GP-100 graphics integrator.


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