Example sentences for: chromogen

How can you use “chromogen” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After washing with PBS, the antibodies were detected using reagents in the Vectastain ABC Elite Kit (Vector Labs, Burlingame, USA); the sections were treated with biotinylated goat anti-rabbit IgG (at 1/200 dilution) for 30 min, rinsed with PBS, incubated with streptavidin-conjugated peroxidase for 30 min, washed with PBS, then treated with the chromogen 3,3'-diaminobenzidine for 5 min.

  • Immunoreactivity of the primary antibody was then labeled either with the chromogen diamino benzidine (DAKO Diagnostika, Hamburg) or with a fluorophore-coupled secondary antibody.

  • Eight consecutive sections from each of the three selected cases were used for a double immunostaining with our published protocols [ 26 27 ] . In brief, all eight sections were first immunostained for SMA with a black chromogen.

  • Section #2 was immunostained with 'melanoma cocktail' using Diaminobenzidine Hydrochloride (DAB) as a chromogen.

  • Slides were developed with a substrate chromogen solution until a brown reaction product was observed, then counterstained with hematoxylin and coverslipped.


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