Example sentences for: chromogen

How can you use “chromogen” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Indirect immunoperoxidase staining was performed at 37°C for one hour using Vector Elite ABC kits (Vector, Burlingame, CA, USA) and diaminobenzidine (Kirkegaard and Perry, Gaitherburg, MD, USA) as a chromogen [ 11 12 ] . The polyclonal antibodies, goat antihuman Ang-1 and Ang-2, and rabbit antihuman Tie1 and Tie2, were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

  • AEC was the chromogen of choice for clean background.

  • Immunoreactivity of the primary antibody was then labeled either with the chromogen diamino benzidine (DAKO Diagnostika, Hamburg) or with a fluorophore-coupled secondary antibody.

  • Finally, after washing the sections in PBS (30 min) and Tris-HCl buffer (pH 7.6)(10 min), the tissue-bound peroxidase was developed by H 2 O 2 using 3, 3' diaminobenzidine as chromogen.

  • The immunoreaction was revealed by a horseradish peroxidase-based chromogen.


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