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Example sentences for: chomczynski

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Total RNA was extracted from two juvenile zebrafish and skeletal muscle of a single adult female using the method of Chomczynski [ 39 ] . The integrity of the RNA was checked by agarose gel electrophoresis.
Total RNA was extracted from the frozen mouse skin biopsies of 30 animals as described by Chomczynski and Sacchi (1987) [ 32 ] . The total RNA was digested by RNase free DNaseI and phenol extracted in order to remove genomic DNA contaminations.
Total RNA was prepared from synovial tissue by an acid phenol method according to the procedure described by Chomczynski and Sacchi [ 31 ] . RNA (20 μg) was digested with five units of DNase in 1× reverse transcription buffer (Gibco/Life Technologies, Grand Island, NY, USA) containing eight units of RNase inhibitor (Gibco/Life Technologies, Grand Island, NY, USA) for 30 minutes at 37°C.
Total RNA was purified from PBMC samples using Clontech Nucleospin RNA II kit reagents (Clontech, Palo Alto, CA) and from whole blood samples using MRC TRI Reagent BD (Molecular Research Center, Cincinnati, OH, USA), an adaptation of the Chomczynski single-step method [ 16 ] , according to the manufacturer's instructions.
Total cellular RNA was isolated from each gastrocnemius muscle by the method of Chomczynski and Sacchi [ 32 ] . RNA preparations were quantified by absorbance at 260 nm and RNA intactness and integrity assessed by ethidium bromide staining after separation by electrophoresis in a 6.6% formaldehyde-1% agarose gel.