Words similar to chemiluminescent
Example sentences for: chemiluminescent
How can you use “chemiluminescent” in a sentence? Here are some example sentences to help you improve your vocabulary:
Bound antibodies were detected by a chemiluminescent detection system (ECL; Amersham Pharmacia Biotech, Piscataway, NJ) as recommended by the manufacturer's protocol . For exposure (first 5 min, and second up to 2 h) we used Kodak XAR film (Eastman Kodak, Rochester, NY).
Samples of known numbers of cells from the cell lines and recombinant, purified Tag prepared from a baculovirus lysate [ 40 ] were loaded on 10% polyacrylamide discontinuous mini-gels (Bio-Rad, Hercules, CA) and electrophoresed conventionally [ 41 ] . Gels were then electrophoretically blotted [ 42 ] onto PVDF membrane (Bio-Rad) without methanol for 15 min at 100 V. Tag was visualized by immunostaining with PAb416 and alkaline phosphatase conjugated secondary antibody (Sigma) using chemiluminescent detection with CDP-Star substrate according to the manufacturers directions (Tropix, Bedford, MA).
The protein was detected using a 1:1000 dilution of rabbit polyclonal antibody generated against recombinant macaque SPAM1 (a generous gift from Dr. James Overstreet, UC Davis) and the WesternBreeze Chemiluminescent Western Blot Immunodetection Kit (Invitrogen, Cat No.
Cyclin E variants in Cdk2 IP were detected by probing with HE12 primary antibody (PharMingen), followed by goat anti-mouse IgG and IgM (Jackson Immunoresearch) secondary antibody conjugated with horse radish peroxidase, and visualized by a chemiluminescent detection system (ECL, Amersham Pharmacia Biotechnology) and autoradiography as indicated above.
Anti-phosphorylated IκB-α polyclonal antibody, IκB monoclonal antibody and chemiluminescent detection reagents were from Cell Signaling Technology (Beverly, MA, USA).
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