Words similar to chemicon
Example sentences for: chemicon
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Following cell thaw, NSs were expanded in suspension culture, and in each case were used between passages 5 and 7. Undifferentiated human NS cells (stage 1) were cultured and expanded in medium containing X-VIVO15 (Cambrex, Walkersville, Maryland, United States), N2 supplement (Invitrogen, Carlsbad, California, United States), 2 μg/ml heparin, 10 ng/ml leukemia inhibitory factor (Chemicon, Temecula, California, United States), 20 ng/ml epidermal growth factor (R&D Systems, Minneapolis, Minnesota, United States), and 20 ng/ml fibroblast growth factor-2 (R&D Systems), and they were frozen after enzymatic dissociation.
Deposition of collagen and elastin in cell and matrix layers was determined by solid-phase ELISA with antibodies against goat anti-collagen I (Chemicon), mouse anti-collagen III (Telios), mouse anti-collagen VI (Telios), or mouse anti-elastin (Sigma), at dilutions recommended by the manufacturers.
Anti-mouse IgG rhodamine conjugate (AP124R) was obtained from Chemicon International (Temecula, CA).
Secondary antibodies were a fluorescein-conjugated goat anti mouse IgG (Boehringer Mannheim, IN) used at a dilution of 1:1000 and a fluorescein-conjugated donkey anti-rabbit IgG (Chemicon, CA) used at a dilution of 1:1000.
7% formaldehyde at -20°C for 20 minutes, then washed twice with 1 × PBS; the coverslips where then incubated with a 1:200 dilution of rabbit anti-cyclin D 1 antibody (Santa Cruz Biotechnology) or control rabbit sera (Biogenex) for 2 hours at room temperature followed by three washes with 1 × PBS; the coverslips were then incubated with a 1:250 dilution of rhodamine-labeled donkey-anti-rabbit anti-sera (Chemicon) for 45 minutes to detect the primary antibody; following three final washes with 1 × PBS, the coverslips were mounted on glass slides using ProLong Antifade (Molecular Probes) for fluorescent detection of GFP and cyclin D 1 on an Olympus IX70 microscope at 100 × magnification.
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