Words similar to chemicon
Example sentences for: chemicon
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Polyclonal antibodies used were MyoD (Santa Cruz Biotechnology, Santa Cruz, California, United States) and nestin (gift from R. McKay); monoclonal antibodies were vimentin, alpha smooth muscle actin, fast-switch myosin, pan-cytokeratin (all from Sigma), and human nuclear antigen (Chemicon, Temecula, California, United States).
We used primary antibodies at the following dilutions: guinea pig anti-insulin, 1:200 (Linco Research, St. Charles, Missouri, United States), mouse anti-β-tubulin III, 1:500 (Sigma); rabbit anti-C-peptide, 1:100 (Linco Research); mouse anti-Nestin, 1:200 (Chemicon); mouse anti-Ki67, 1:100 (Novocastra, Newcastle, United Kingdom); rabbit anti–cleaved caspase-3, 1:200 (Cell Signaling, Beverly, Massachusetts, United States); rabbit anti-glucagon, 1:200 (Dako, Carpinteria, California, United States); rabbit anti-Glut-2, 1:200 (ADI, San Antonio, Texas, United States); mouse anti-proinsulin, 1:500 (O.
Monoclonal antibodies to OPN, to TYRP-1 and to TSP-1 were purchased commercially (OPN [1:100], Chemicon International, Inc., Temecula, CA, USA; TYRP-1 [1:80, clone G-17], Santa Cruz Biotechnology, Inc., CA, USA; TSP-1 [1:320], BD Transduction Laboratories, CA, USA).
Sections from the DRG were processed for the visualization of MIF and substance P as follows: sections were exposed to anti-MIF (1:200) and anti-substance P (guinea pig polyclonal; 1:1000; Chemicon; Tamecula, CA) overnight at 4°C.
Immunohistochemistry was performed using antibodies to ACTH (rabbit polyclonal anti-ACTH; Chemicon International) or glial fibrillary acid protein (rabbit polyclonal anti-GFAP; Chemicon International).
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