Words similar to chemicon
Example sentences for: chemicon
How can you use “chemicon” in a sentence? Here are some example sentences to help you improve your vocabulary:
MIF concentrations within cell lysates were determined using a rat MIF ELISA kit (Chemicon).
Monoclonal antibodies to OPN, to TYRP-1 and to TSP-1 were purchased commercially (OPN [1:100], Chemicon International, Inc., Temecula, CA, USA; TYRP-1 [1:80, clone G-17], Santa Cruz Biotechnology, Inc., CA, USA; TSP-1 [1:320], BD Transduction Laboratories, CA, USA).
The cells were stained with a FITC labelled antibody directed against the major immediate early antigen (MIE) (Chemicon, Temecula, CA) and visualized using a fluorescent microscope.
Following cell thaw, NSs were expanded in suspension culture, and in each case were used between passages 5 and 7. Undifferentiated human NS cells (stage 1) were cultured and expanded in medium containing X-VIVO15 (Cambrex, Walkersville, Maryland, United States), N2 supplement (Invitrogen, Carlsbad, California, United States), 2 μg/ml heparin, 10 ng/ml leukemia inhibitory factor (Chemicon, Temecula, California, United States), 20 ng/ml epidermal growth factor (R&D Systems, Minneapolis, Minnesota, United States), and 20 ng/ml fibroblast growth factor-2 (R&D Systems), and they were frozen after enzymatic dissociation.
The protein was soluble, and was purified by standard Ni-chelation chromatography [ 45 ] . Purified recombinant profilin and antibodies against profilin, RSV P protein, and F protein have been described previously [ 11 25 ] . Polyclonal antibodies to RSV proteins G, N, and M were purchased from Chemicon International (Temecula, CA) and the corresponding rhodamine-conjugated secondary antibody from Sigma.
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