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Primers were designed with the Primer3 software http://www-genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgiand were synthesized by Sigma-Genosys.
The Primer 3 program http://www-genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgiwas used to select PCR primers.
Intronic oligonucleotide primer pairs flanking the coding fragments were designed for both the genes using program primer 3 http://www.genome.wi.mit.edu/cgi-bin/primer/primer3.cgiusing the genomic sequences of SLC15A1 and GPC5 gene respectively.
Once a sequence containing the desired polymorphism was selected (usually 400-700 bp in length), it was run through the Repeat Masker program http://ftp.genome.washington.edu/cgi-bin/RepeatMaskerin order to avoid selecting PCR primers within Alu, L1, or other repeats.
A web-interfaced program, Primer3 http://www-genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgi, was used.