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Example sentences for: cgi-bin
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Once a sequence containing the desired polymorphism was selected (usually 400-700 bp in length), it was run through the Repeat Masker program http://ftp.genome.washington.edu/cgi-bin/RepeatMaskerin order to avoid selecting PCR primers within Alu, L1, or other repeats.
A user interface is available at http://gene.cpmc.columbia.edu/cgi-bin/gene.cgi.
A web-interfaced program, Primer3 http://www-genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgi, was used.
The Primer 3 program http://www-genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgiwas used to select PCR primers.
Primers used for amplification of BMP-5 cDNA were designed to unique sequences of rat BMP-5 using the Primer3 program http://www-genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgi.