Example sentences for: cetus

How can you use “cetus” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Total RNA was purified from dissected nephron segments using Tri Reagent (Molecular Research Center) and reverse-transcribed to single-stranded cDNA using Moloney murine leukemia virus reverse transcriptase and 2.5 μM of random hexamers according to the manufacturer's protocol (GeneAmp RNA PCR kit, Perkin Elmer Cetus, Norwalk, CT).

  • 3), 5 mM KCl, 1.5 mM MgCl 2 , 200 μM of each dNTP, and 2.5 units of Amplitaq (Perkin-Elmer/Cetus).

  • PCR reactions were carried out in 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 2.5 mM MgCl 2 , 0.2 mM dNTPs and 1 μM primers at 94°C for 0.5 min, 60°C for 0.5 min, and 72°C for 1.0 min for 35 cycles in a Perkin Elmer Cetus 9600 thermal cycler.

  • Amplifications were done using either a Perkin-Elmer Amp PCR System 2400 or Model 480 DNA Thermal Cycler (Perkin-Elmer Cetus, Norwalk, Conn).

  • Reaction conditions were as follows for a 50 μl PCR reaction : Perkin-Elmer Cetus PCR buffer; 1.5 mM MgCl 2 , 0.25 mM dNTPs, 1.5 μl of each primer (10 μM), 1% DMSO, 5 U of Taq polymerase (Life Technologies).


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