Words similar to cetus
Example sentences for: cetus
How can you use “cetus” in a sentence? Here are some example sentences to help you improve your vocabulary:
PCRs were performed in a model 9600 Thermocycler (Perkin-Elmer Cetus) using the following cycling protocol: initial denaturation at 94°C for 10 min; 94.
Each 100 μl PCR contained 3-5 μl of template DNA, 5 μl of dimethyl sulfoxide (DMSO), and 90-92 μl of a reaction mixture (200 μM (each) deoxynucleotide triphosphates, 1.0 μM (each) primer, 1.25 U of AmpliTaq DNA Polymerase (Perkin-Elmer Cetus), 10 mM Tris hydrochloride pH 8.3, 50 mM KCl, 1.5 mM MgCl 2 , and 0.01% (w/v) gelatin).
Total RNA was purified from dissected nephron segments using Tri Reagent (Molecular Research Center) and reverse-transcribed to single-stranded cDNA using Moloney murine leukemia virus reverse transcriptase and 2.5 μM of random hexamers according to the manufacturer's protocol (GeneAmp RNA PCR kit, Perkin Elmer Cetus, Norwalk, CT).
The sources of materials used in this study: Phagescript and pBS(-) plasmid were from Stratagene; Taq polymerase and the Stoffel fragment of Taq were from Perkin Elmer Cetus; gp32 was from Pharmacia; Bst E II-digested Lambda DNA was from New England Biolabs.
PCR reactions (10 mM Tris.HCl, pH 8.3, 5 mM KCl, 1.5 mM MgCl 2 , 200 μM of each deoxyribonucleoside triphosphate, each primer at 2 μM, and 2.5 units of Amplitaq (Perkin-Elmer/Cetus) involved 35 cycles of denaturation (95°C for 1.0 min), primer annealing (55°C for 1.0 min), and primer extension (72°C for 2 min).
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