Example sentences for: cetus

How can you use “cetus” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Reaction conditions were as follows for a 50 μl PCR reaction : Perkin-Elmer Cetus PCR buffer; 1.5 mM MgCl 2 , 0.25 mM dNTPs, 1.5 μl of each primer (10 μM), 1% DMSO, 5 U of Taq polymerase (Life Technologies).

  • Amplifications were done using either a Perkin-Elmer Amp PCR System 2400 or Model 480 DNA Thermal Cycler (Perkin-Elmer Cetus, Norwalk, Conn).

  • Each 100 μl PCR contained 3-5 μl of template DNA, 5 μl of dimethyl sulfoxide (DMSO), and 90-92 μl of a reaction mixture (200 μM (each) deoxynucleotide triphosphates, 1.0 μM (each) primer, 1.25 U of AmpliTaq DNA Polymerase (Perkin-Elmer Cetus), 10 mM Tris hydrochloride pH 8.3, 50 mM KCl, 1.5 mM MgCl 2 , and 0.01% (w/v) gelatin).

  • The sources of materials used in this study: Phagescript and pBS(-) plasmid were from Stratagene; Taq polymerase and the Stoffel fragment of Taq were from Perkin Elmer Cetus; gp32 was from Pharmacia; Bst E II-digested Lambda DNA was from New England Biolabs.

  • Total RNA was purified from dissected nephron segments using Tri Reagent (Molecular Research Center) and reverse-transcribed to single-stranded cDNA using Moloney murine leukemia virus reverse transcriptase and 2.5 μM of random hexamers according to the manufacturer's protocol (GeneAmp RNA PCR kit, Perkin Elmer Cetus, Norwalk, CT).


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