Words similar to cetus
Example sentences for: cetus
How can you use “cetus” in a sentence? Here are some example sentences to help you improve your vocabulary:
Each 100 μl PCR contained 3-5 μl of template DNA, 5 μl of dimethyl sulfoxide (DMSO), and 90-92 μl of a reaction mixture (200 μM (each) deoxynucleotide triphosphates, 1.0 μM (each) primer, 1.25 U of AmpliTaq DNA Polymerase (Perkin-Elmer Cetus), 10 mM Tris hydrochloride pH 8.3, 50 mM KCl, 1.5 mM MgCl 2 , and 0.01% (w/v) gelatin).
Amplifications were done using either a Perkin-Elmer Amp PCR System 2400 or Model 480 DNA Thermal Cycler (Perkin-Elmer Cetus, Norwalk, Conn).
The sources of materials used in this study: Phagescript and pBS(-) plasmid were from Stratagene; Taq polymerase and the Stoffel fragment of Taq were from Perkin Elmer Cetus; gp32 was from Pharmacia; Bst E II-digested Lambda DNA was from New England Biolabs.
PCR reactions were carried out in 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 2.5 mM MgCl 2 , 0.2 mM dNTPs and 1 μM primers at 94°C for 0.5 min, 60°C for 0.5 min, and 72°C for 1.0 min for 35 cycles in a Perkin Elmer Cetus 9600 thermal cycler.
3), 5 mM KCl, 1.5 mM MgCl 2 , 200 μM of each dNTP, and 2.5 units of Amplitaq (Perkin-Elmer/Cetus).
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