Example sentences for: cet

How can you use “cet” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Based on these findings, and the presence of a Cet1 homolog in the Apergillus fumigatus proteome, we conclude that RNA triphosphatase is a valid target for antifungal drug development.

  • The deleted segment contains the catalytic domain essential for triphosphatase activity in vitro and for complementation of the cet1Δ strain of S. cerevisiae [ 10 11 ] . Second, we introduced the linearized deletion allele into a diploid C. albicans ura3/ura3 arg4/arg4 strain and selected for Arg +transformants.

  • Searches of public genome databases indicate that Aspergillus fumigatus (a major invasive pathogen in humans, with severe morbidity and mortality) encodes a homolog of Cet1, as does Neurospora crassa.

  • This is not a straw-man issue, given that S. cerevisiae encodes two homologous RNA triphosphatases (Cet1 and Cth1), of which only Cet1 is essential for capping and cell viability [ 8 15 ] . We use classical genetic approaches to show that the respective genes encoding RNA triphosphatase and RNA guanylyltransferase are essential in S. pombe.

  • To address these issues, we have characterized the RNA triphosphatases of two other fungi, including the human pathogen Candida albicans and the fission yeast Schizosaccharomyces pombe [ 10 11 12 ] . The fungal triphosphatases, S. cerevisiae Cet1, C. albicans CaCet1 and S. pombe Pct1, belong to a new family of metal-dependent phosphohydrolases that embraces the triphosphatase components of DNA virus and protozoan mRNA capping systems [ 1 7 13 14 ] . The defining features of the metal-dependent RNA triphosphatases are two glutamate-containing motifs that are required for catalysis and comprise the metal-binding site in the crystal structure of S. cerevisiae Cet1.


How many words do you know? Try our free vocabulary size test!


Search

Search for example sentences

Loading Loading...
Quantcast