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Example sentences for: ccaf

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We have previously determined, using dose-dependent studies, that this concentration is optimal for functions of this chemokine on these embryonic fibroblasts and in processes of granulation tissue formation [ 3 4 ] ). Although chemokine concentrations of 10 -2-10 ng/ml can chemoattract and activate leukocytes, chemokines acting on other cell types such as endothelial cells, smooth muscle cells and fibroblasts require concentrations in the range of 10 2ng/ml and higher [ 4 26 27 28 ] . This is within physiological range; for example wound fluid from burn patients contain MGSA (a chemokine that is highly homologous to cCAF) concentrations of 10 2-10 3ng/ml [ 29 ] . Because both the N-terminal and C-terminal peptides of cCAF have been shown to be functional in culture and in vivo (1-4, 30), we also tested these two peptides (Fig.
Our results strongly suggest that cCAF plays an important role in wound repair by activating fibroblasts to form early granulation tissue, through cell migration, myofibroblast differentiation and production/accumulation of ECM molecules that are critical for proper development of the healing tissue of wounds.
The FN mRNA levels are not affected by cCAF treatment but the protein accumulates in culture and in the granulation tissue of wounds upon exposure to the chemokine.
cCAF was then added at various doses (0-1000 ng/ml).
Placing cCAF in both wells abrogated cell migration, strongly suggesting that this protein chemoattracts fibroblasts (Fig 8B).