Words similar to carboxy-terminus
Example sentences for: carboxy-terminus
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Each clone containing an in-frame insertion should encode a truncated α s protein with GFP at the carboxy-terminus due to a stop codon in the Kan r . Thirty-five of the PCR-positive clones were transiently expressed in HEK 293 cells, and 13 were fluorescent.
To determine what effect the GFP insertions had on α s localization, the full-length fusion proteins were transiently co-expressed in HEK 293 cells with G protein subunits β 1 and γ 7 , which have been shown to mediate signaling between the β-adrenergic receptor and G s [ 16 ] . Amino- and carboxy-terminus GFP fusions, α s -GFP(N) and α s -GFP(C), respectively, were also co-expressed with β 1 and γ 7 for comparison.
This basic domain organization shows a striking parallel to that of magnesium chelatase, a heterotrimeric enzyme containing BchD, BchI and BchH subunits, that performs ATP-dependent insertion of Mg 2+into the protoporphyrin IX ring in the course of chlorophyll biosynthesis [ 49 ] . In particular, the BchD subunit resembles midasin in possessing a single AAA protomer close to its NH 2 -terminus, together with a short aspartate-glutamate-rich region and a MIDAS-containing domain at its carboxy-terminus.
the intracellular carboxy-terminus region or the amino terminus between amino acids 210 and 330).
Kinesins are characterized by a conserved motor domain of approximately 350 amino acids that may be located at the amino terminus (KIN-N), carboxy-terminus (KIN-C) or within the polypeptide (KIN-I) [ 27 ] and the motor domain of KIF27 is located in the N-terminus of the protein.