Words similar to c-crk
Example sentences for: c-crk
How can you use “c-crk” in a sentence? Here are some example sentences to help you improve your vocabulary:
Thus c-crk interacts constitutively with downstream effectors such as C3G via its SH3 domains, and interacts in a phosphotyrosine dependent fashion with tyrosine phosphorylated receptors using its SH2 domain [ 39 ] . To analyze downstream signals regulated by these adaptor proteins, investigators have developed methods that target them to distinct cellular membrane compartments by using heterologous targeting sequences.
These data suggest that the tyrosine phosphorylated Cas chimera perturbs signaling via v-crk and probably via endogenous c-crk to affect the activation of the JNK pathway.
Adaptor proteins that regulate tyrosine kinase signaling pathways fall into two major categories, those primarily with protein/protein interaction domains such as SH2, SH3, PTB, and PH domains, exemplified by the adaptor proteins c-crk, nck and grb-2, and those that are primarily tyrosine phosphorylated, exemplified by proteins such as Cas, Dok, IRS-1 and 2, Slp-76, LAT, TRIM or SIT.
To determine if the tyrosine phosphorylated chimera could compete with endogenous Cas for binding to endogenous c-crk, we transfected either the isolated attenuated Src kinase domain as control, or the tyrosine phosphorylated Cas substrate domain (Src*/Cas(SD)) into Cos-7 cells, and immunoprecipitated endogenous c-crk (Fig.
As the tyrosine phosphorylated Cas substrate domain acts as a dominant negative for v-crk mediated transformation and JNK activation, and can also interact with v-crk and c-crk (data not shown), we wanted to determine if it could compete for binding of v- and c-crk to endogenous Cas.
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