Example sentences for: btbd
How can you use “btbd” in a sentence? Here are some example sentences to help you improve your vocabulary:
The sense primers for amplification of truncated BTBD2 fragments were: U1 (504-525aa) 5'-GACCCCGGGCTACGCGGCCGGGAACAACAATG-3', U2 (460-525aa) 5'-GACCCCGGGGGTGCTGCCCAACGTCAACTACAC-3', U3 (404-525aa) 5'-GACCCCGGGCGTGGTGGGATTTGGGCTGTATGG-3', U4 (289-525aa) 5'-GACCCCGGGCCGCTGGTCCGAGGCCGAGTGTC-3'.
The fragment of TOP1 from amino acid 215 to 331 was capable of binding to BTBD2.
To determine whether the mRNA contained the BTBD2 gene sequences directly upstream of the longest cDNA clone, RT-PCR was used with a sense primer complementary to the predicted start codon.
To determine if this sequence might be part of the BTBD2 mRNA, RT-PCR followed by nested PCR was used.
C-terminal deletions of either protein eliminated binding to TOP1 (C-terminal truncation of 112 aa of BTBD1 or 33 aa of BTBD2 in the two-hybrid assay; or a C-terminal truncation of 40 aa of BTBD2 in GST-pulldown).
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