Example sentences for: btbd
How can you use “btbd” in a sentence? Here are some example sentences to help you improve your vocabulary:
6B, m-BTBD2F).
To construct the expression plasmid encoding C-terminal-myc-tagged truncated BTBD2 having the N-terminal half the BTB domain deleted (m-BTBD2T), the coding region of BTBD2 from the second ATG to almost the end of the 3'-UTR (445-2542 bp) was amplified with primers (Upper primer: 5'-ATGTTCAACGGGGGAATGGCCACAACATC-3', Lower primer: 5'-GGGGAGGACGCAGGTTCCAGGAGT-3') containing BamH I and Hind III cleavage sites and inserted into pCMV-Tag 3 vector (Stratagene) cut with BamH I and Hind III.
The region of TOP1 sufficient for binding BTBD2 was narrowed to residues 215 - 329 in the "cap" region (core subdomain II) [ 31 ] . Catalytic activities of TOP1 were affected slightly or not at all by co-incubation with GST-BTBD2 suggesting that the interaction does not regulate enzyme activity directly.
BTBD1 mRNA was highly expressed in testes, heart and skeletal muscle.
Two-hybrid analysis was also used to map regions of the TOP1 core domain that interact with BTBD2.