Example sentences for: btbd
How can you use “btbd” in a sentence? Here are some example sentences to help you improve your vocabulary:
To determine whether the mRNA contained the BTBD2 gene sequences directly upstream of the longest cDNA clone, RT-PCR was used with a sense primer complementary to the predicted start codon.
The sense primer sequences are as follows: BTBD1: U1 (395-482aa) 5'-GACCCCGGGGAATGATACCGGCTTTAGTTGTGA-3', U2 (342-482aa) 5'-GACCCCGGGCTGGGGTTACAGTGGGACGAGT-3', U3 (213-482aa) 5'-GACCCCGGGAAGTGCAGAAGGGTTTACT-3', U4 (303-482aa) 5'-GACCCCGGGAAACCTCTTTCTTCATTTTACTGT-3', The same antisense primer was used for all amplifications: 5'-CGGAATTCTTTGTGGCCATTTATGTTTTTGAC-3'.
BTBD2 was more highly expressed in skeletal muscle.
Poly (A)+RNA from human tissues (2 μg/lane) was probed with BTBD1, BTBD2 or TOP1 cDNA.
The longest BTBD2 cDNA obtained from two-hybrid screening (2413 bp) contained an ORF starting at the 5' end.
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