Example sentences for: btb
How can you use “btb” in a sentence? Here are some example sentences to help you improve your vocabulary:
Truncation of the BTB domains demonstrated that they were not required for interaction with TOP1.
• Less than the C-terminal half of each BTBD protein lacking the BTB domain is sufficient for binding to TOP1.
aa, amino acid; BTB: Broad-Complex, Tramtrack and Bric-a-brac; GST, glutathione-S-transferase; NLS, nuclear localization signal; ORF, open reading frame; RT-PCR, reverse transcription - polymerase chain reaction; TOP1, DNA topoisomerase I.
The BTBD proteins contain an amino terminal BTB/POZ domain as well as a C-terminal region that is related to proteins that contain the kelch domain.
To construct the expression plasmid encoding C-terminal-myc-tagged truncated BTBD2 having the N-terminal half the BTB domain deleted (m-BTBD2T), the coding region of BTBD2 from the second ATG to almost the end of the 3'-UTR (445-2542 bp) was amplified with primers (Upper primer: 5'-ATGTTCAACGGGGGAATGGCCACAACATC-3', Lower primer: 5'-GGGGAGGACGCAGGTTCCAGGAGT-3') containing BamH I and Hind III cleavage sites and inserted into pCMV-Tag 3 vector (Stratagene) cut with BamH I and Hind III.
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