Example sentences for: btb
How can you use “btb” in a sentence? Here are some example sentences to help you improve your vocabulary:
Truncation of the BTB domains demonstrated that they were not required for interaction with TOP1.
The BTB domain is a highly conserved 120 amino acid, hydrophobic-rich, protein-protein interaction domain that mediates homodimerization and heterodimerization of a large number of proteins with diverse functions [ 9 ] . The three dimensional structure of one BTB domain [ 33 ] has been characterized through structure-function studies [ 34 ] . Proteins containing BTB domains are currently categorized into three groups [ 35 ] : 1) DNA-binding proteins often containing zinc fingers in the C-terminal region, 2) actin binding proteins that contain a kelch repeat of ~50 residues that terminate in a pair of glycine residues [ 36 ] and 3) proteins that have neither an actin-binding domain nor a DNA-binding domain.
• Less than the C-terminal half of each BTBD protein lacking the BTB domain is sufficient for binding to TOP1.
To determine the localization of BTBD2 in cells, we transiently transfected HeLa cells with expression plasmids encoding either myc-tagged full length BTBD2 (m-BTBD2F) or a truncated BTBD2 having the N-terminal half of the BTB domain removed (m-BTBD2T).
aa, amino acid; BTB: Broad-Complex, Tramtrack and Bric-a-brac; GST, glutathione-S-transferase; NLS, nuclear localization signal; ORF, open reading frame; RT-PCR, reverse transcription - polymerase chain reaction; TOP1, DNA topoisomerase I.
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