Example sentences for: btb
How can you use “btb” in a sentence? Here are some example sentences to help you improve your vocabulary:
To construct the expression plasmid encoding C-terminal-myc-tagged truncated BTBD2 having the N-terminal half the BTB domain deleted (m-BTBD2T), the coding region of BTBD2 from the second ATG to almost the end of the 3'-UTR (445-2542 bp) was amplified with primers (Upper primer: 5'-ATGTTCAACGGGGGAATGGCCACAACATC-3', Lower primer: 5'-GGGGAGGACGCAGGTTCCAGGAGT-3') containing BamH I and Hind III cleavage sites and inserted into pCMV-Tag 3 vector (Stratagene) cut with BamH I and Hind III.
• Less than the C-terminal half of each BTBD protein lacking the BTB domain is sufficient for binding to TOP1.
Truncation of the BTB domains demonstrated that they were not required for interaction with TOP1.
The BTB domain is a highly conserved 120 amino acid, hydrophobic-rich, protein-protein interaction domain that mediates homodimerization and heterodimerization of a large number of proteins with diverse functions [ 9 ] . The three dimensional structure of one BTB domain [ 33 ] has been characterized through structure-function studies [ 34 ] . Proteins containing BTB domains are currently categorized into three groups [ 35 ] : 1) DNA-binding proteins often containing zinc fingers in the C-terminal region, 2) actin binding proteins that contain a kelch repeat of ~50 residues that terminate in a pair of glycine residues [ 36 ] and 3) proteins that have neither an actin-binding domain nor a DNA-binding domain.
The BTBD proteins contain an amino terminal BTB/POZ domain as well as a C-terminal region that is related to proteins that contain the kelch domain.
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