Example sentences for: bromphenol

How can you use “bromphenol” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Samples containing 60 μg of total protein were mixed with an equal volume of 2x SDS-Laemmli sample buffer (100 mM Tris-HCl, pH 6.8, 20% glycerol, 200 mM dithiothreitol, 4% SDS, and 0.2% bromphenol blue) and boiled for 5 min, then resolved using either 12% SDS-polyacrylamide gel electrophoresis.

  • 8, 2% SDS, 0.1% bromphenol blue, 0.5% β-mercaptoethanol, 20% glycerol).

  • After determination of the protein concentration, lysates were mixed with an equal volume of 2 × gel sample buffer containing 6% sodium dodecyl sulfate, 20% glycerol, 10% β-mercaptoethanol, 0.02% bromphenol blue and Complete Protease Inhibitor cocktail.

  • Equal amounts of protein lysates from each condition in 1X sample buffer (125 mM Tris-HCl, pH 6.8, 1% β-mercaptoethanol, 2% SDS, 5% glycerol, 0.003% bromphenol blue) were separated on 7.5 or 10% polyacrylamide gels and subsequently transferred to nitrocellulose membranes.

  • Both the supernatant and the pellet were diluted by adding 6X sample buffer (10% SDS, 1.2 mg/mL bromphenol blue, 0.6 M DTT, 30% glycerol, .1 M Tris/HCl pH 6.8) and heated to 80°C for 15 minutes to denature the protein.


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