Words similar to bromphenol

Example sentences for: bromphenol

How can you use “bromphenol” in a sentence? Here are some example sentences to help you improve your vocabulary:

Both the supernatant and the pellet were diluted by adding 6X sample buffer (10% SDS, 1.2 mg/mL bromphenol blue, 0.6 M DTT, 30% glycerol, .1 M Tris/HCl pH 6.8) and heated to 80°C for 15 minutes to denature the protein.
Samples containing 60 μg of total protein were mixed with an equal volume of 2x SDS-Laemmli sample buffer (100 mM Tris-HCl, pH 6.8, 20% glycerol, 200 mM dithiothreitol, 4% SDS, and 0.2% bromphenol blue) and boiled for 5 min, then resolved using either 12% SDS-polyacrylamide gel electrophoresis.
For Western blot analysis, 15 μl of samples containing 20 μg protein were mixed with equal volume of 2 × loading buffer (1X = 125 mM TRIS-HCl, pH 6.8; 2% SDS, 5% glycerol, 0.003% bromphenol blue and 1% 1-mercaptoethanol), and boiled for 5 min.
Equal amounts of protein lysates from each condition in 1X sample buffer (125 mM Tris-HCl, pH 6.8, 1% β-mercaptoethanol, 2% SDS, 5% glycerol, 0.003% bromphenol blue) were separated on 7.5 or 10% polyacrylamide gels and subsequently transferred to nitrocellulose membranes.
4 mM 2-mercaptoethanol, 0.1% bromphenol blue, and 1 mM EGTA) then diluted 1:1 in PBS.