Words similar to bromo-
Example sentences for: bromo-
How can you use “bromo-” in a sentence? Here are some example sentences to help you improve your vocabulary:
Ten μm thick frozen sections were prepared and stained with 5-bromo-4-chloro-3-indolyl-β-galactopyranoside (X-gal) solution (Molecular Probes, The Netherlands) overnight at 37°C.
Alkaline phosphatase-conjugated secondary antibody (goat anti-rabbit immunoglobulin G; Pierce) was used to visualize proteins by development with nitroblue tetrazolium and 5-bromo-4-chloro-3-indolylphosphate (NBT-BCIP; Fisher Scientific, Fair Lawn, NJ).
From the pooled stock of the transformants, ten million cells were screened on U -H -W -L -galactose plates containing X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactosidase).
Excess secondary antibody was removed by three 5-min washes with TNT buffer and color was developed using the 5-bromo-4-chloro-3-indolylphosphate/nitro blue tetrazolium (BCIP/NBT) alkaline phosphatase substrate.
Then, cells were fixed with 0.5% glutaraldehyde and stained for β-galactosidase (LacZ) activity using 5-bromo-4-chloro-3-indoyl-β- D-galactopyronoside (X-Gal) as the substrate.