Words similar to bromophenol
Example sentences for: bromophenol
How can you use “bromophenol” in a sentence? Here are some example sentences to help you improve your vocabulary:
At this time a 4.5 μl aliquot was removed for a zero time point and quenched with 5 μl stop dye (95% formamide, 20 mM EDTA, 0.5% xylene cyanol, and 0.5% bromophenol blue).
Reactions were stopped by addition of loading buffer: 80% formamide, 10 mM Na 2 EDTA (pH 8.0), and 1 mg/ml each bromophenol blue and xylene cyanol.
Cells were homogenized in a glass-teflon homogenizer in a minimal volume of PBS and diluted 1:1 (vol:vol) with SDS-gel loading buffer composed 100 mM Tris-Cl (pH 6.8), 200 mM dithiothreitol, 4% SDS, 0.2% bromophenol blue and 20% glycerol.
Gel was run at 15 mAmps constant current until the leading dye band (bromophenol blue) migrated near the bottom.
Following incubation of the mixture for 10 min at 4°C, equal volume of 2× gel-loading dye containing 0.25% bromophenol blue and 20% glycerol was added.