Words similar to bromophenol
Example sentences for: bromophenol
How can you use “bromophenol” in a sentence? Here are some example sentences to help you improve your vocabulary:
Following incubation of the mixture for 10 min at 4°C, equal volume of 2× gel-loading dye containing 0.25% bromophenol blue and 20% glycerol was added.
The RNA was denatured prior to loading by incubating the RNA at 65°C for 10 min in a loading buffer comprising 1X MOPS, 50% formamide, 6.5% formaldehyde, 5% glycerol, 0.1 mM EDTA, 0.025% bromophenol blue, 0.025% xylene cyanol.
At the end of the differentiation protocol (day 14), additional plates were stained to detect foci formation as follows: cells were washed with 1 × PBS, fixed in methanol for 1 minute, stained with bromophenol blue for 1 minute, then washed again with 1 × PBS.
Total RNA was prepared from W303 and WHT4-1 yeast cells using hot acid phenol extraction [ 41 ] . For electrophoresis, 20 μg of RNA per sample was resolved on a 1% agarose gel containing 2.2 M formaldehyde at 5V/cm until the bromophenol blue migrated 8 cm.
The amplified PCR products (20 μl total) were mixed with 10 μl of loading buffer (95% formamide, 10 mM EDTA, 0.1% xylene cyanol, 0.1% bromophenol blue) and 5 μl aliquots were applied to a 5% denaturing polyacrylamide gel.