Words similar to bromophenol
Example sentences for: bromophenol
How can you use “bromophenol” in a sentence? Here are some example sentences to help you improve your vocabulary:
Total RNA was prepared from W303 and WHT4-1 yeast cells using hot acid phenol extraction [ 41 ] . For electrophoresis, 20 μg of RNA per sample was resolved on a 1% agarose gel containing 2.2 M formaldehyde at 5V/cm until the bromophenol blue migrated 8 cm.
The amplified PCR products (20 μl total) were mixed with 10 μl of loading buffer (95% formamide, 10 mM EDTA, 0.1% xylene cyanol, 0.1% bromophenol blue) and 5 μl aliquots were applied to a 5% denaturing polyacrylamide gel.
8, 2% SDS, 20% glycerol, 0.1% bromophenol blue), and heated for 5 min at 95°C.
[ 17 ] Briefly, the PCR products (4 μl) were mixed with SSCP sample buffer (6 μl, formamide with 0.05% bromophenol blue and xylene cyanol).
Cells were homogenized in a glass-teflon homogenizer in a minimal volume of PBS and diluted 1:1 (vol:vol) with SDS-gel loading buffer composed 100 mM Tris-Cl (pH 6.8), 200 mM dithiothreitol, 4% SDS, 0.2% bromophenol blue and 20% glycerol.