Example sentences for: bromophenol

How can you use “bromophenol” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 8, 2% SDS, 20% glycerol, 0.1% bromophenol blue), and heated for 5 min at 95°C.

  • Cells were homogenized in a glass-teflon homogenizer in a minimal volume of PBS and diluted 1:1 (vol:vol) with SDS-gel loading buffer composed 100 mM Tris-Cl (pH 6.8), 200 mM dithiothreitol, 4% SDS, 0.2% bromophenol blue and 20% glycerol.

  • The amplified PCR products (20 μl total) were mixed with 10 μl of loading buffer (95% formamide, 10 mM EDTA, 0.1% xylene cyanol, 0.1% bromophenol blue) and 5 μl aliquots were applied to a 5% denaturing polyacrylamide gel.

  • At the end of the differentiation protocol (day 14), additional plates were stained to detect foci formation as follows: cells were washed with 1 × PBS, fixed in methanol for 1 minute, stained with bromophenol blue for 1 minute, then washed again with 1 × PBS.

  • [ 17 ] Briefly, the PCR products (4 μl) were mixed with SSCP sample buffer (6 μl, formamide with 0.05% bromophenol blue and xylene cyanol).


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