Example sentences for: bromophenol

How can you use “bromophenol” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Fifteen micrograms of protein were added to 4X loading buffer (250 mM Tris pH 6.8, 8% SDS, 20% glycerol, 0.012% bromophenol blue, 4% β-mercaptoethanol), heated to 95°C for 5 minutes, electrophoresed in 12.

  • The amplified PCR products (20 μl total) were mixed with 10 μl of loading buffer (95% formamide, 10 mM EDTA, 0.1% xylene cyanol, 0.1% bromophenol blue) and 5 μl aliquots were applied to a 5% denaturing polyacrylamide gel.

  • The RNA was denatured prior to loading by incubating the RNA at 65°C for 10 min in a loading buffer comprising 1X MOPS, 50% formamide, 6.5% formaldehyde, 5% glycerol, 0.1 mM EDTA, 0.025% bromophenol blue, 0.025% xylene cyanol.

  • Cells were homogenized in a glass-teflon homogenizer in a minimal volume of PBS and diluted 1:1 (vol:vol) with SDS-gel loading buffer composed 100 mM Tris-Cl (pH 6.8), 200 mM dithiothreitol, 4% SDS, 0.2% bromophenol blue and 20% glycerol.

  • Matched samples from the proteolysis reaction were quenched with trichloroacetic acid (10%, w/v), protein was sedimented, then boiled in (75 mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 0.1% 2-mercaptoethanol and 0.01% bromophenol blue), subjected to SDS-PAGE using 10 or 15% resolving gels, and protein bands visualized by Coomassie staining.


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