Words similar to blunt-ended

Example sentences for: blunt-ended

How can you use “blunt-ended” in a sentence? Here are some example sentences to help you improve your vocabulary:

1) The ble gene was PCR amplified from pPICZalphaC as a 375-bp fragment using primers Ble-N and Ble-C; the amplicon was blunt-ended and ligated into the blunt-cloning site of pT7Blue to generate pT7Blue-bleA.
The resulting plasmid was then cut with Eco RI, blunt-ended with Klenow polymerase and dNTPs, and self-religated, in order to destroy the Eco RI site at the boundary between core ars3002 and the enhancer that was regenerated during the process of Cla I linker addition.
1) was cut and blunt-ended by treatment with Klenow polymerase (New England Biolabs) and dNTPs.