Words similar to blunt-end

Example sentences for: blunt-end

How can you use “blunt-end” in a sentence? Here are some example sentences to help you improve your vocabulary:

A blunt-end linker oligomer was designed according to Mueller and Wold [ 55 ] , and was formed by annealing a mixture of oligomers 7A and 7B, 20 μm each, in DNAb/50, by first heating in a 100° temperature block for 1 minute, and then allowing the block to cool to room temperature, sitting on the bench top.
The 5' and 3' primers contained Nde I and Bam HI restriction sites, respectively, and were designed to amplify the rec12 +cDNA from the first ATG in exon 1 to a position +142 bp downstream of the stop codon in exon 5. RT-PCR products were cloned by blunt-end ligation into pCR-Blunt (Invitrogen Corp.) and both strands were subject to DNA sequencing (GenBank accession no.
For DNA fragments generated by ChIP (or blunt-end restriction) these limitations are effectively overcome by prior phosphatase treatment, by using recombinant terminal transferase enzyme, and by spiking a small quantity of terminating nucleotide into the tailing reaction.