Words similar to blunt-cloning

Example sentences for: blunt-cloning

How can you use “blunt-cloning” in a sentence? Here are some example sentences to help you improve your vocabulary:

1) The ble gene was PCR amplified from pPICZalphaC as a 375-bp fragment using primers Ble-N and Ble-C; the amplicon was blunt-ended and ligated into the blunt-cloning site of pT7Blue to generate pT7Blue-bleA.
1) A 757-bp fragment containing 246 bp of the 3' end of the M. smegmatis sigE gene and 511 bp of the 5' end of the adjacent ORF1 gene was PCR amplified using primer 7208 (which contains a Hpa I restriction site) and primer 7209 and cloned into the Eco RV blunt-cloning site of pT7Blue to generate pSEKO.