Words similar to bioluminescent
Example sentences for: bioluminescent
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Linear as well as nonlinear trends in the data come into play in experiments using luciferase reporter technology [ 19 20 ] . Such artifacts were shown (in one substrate replenishment experiment) to be the result of inevitable depletion of luciferin from the medium surrounding the tissues over time [ 19 ] . Removal of these trends quantitatively clarifies the record of bioluminescent activity with regard to any rhythmicity that might be present [ 27 ] . The normalization procedure we use (see Figure 1and [ 27 ] for more detail) exposes the rhythm (if one is present) in a manner that is quantifiable without reference to the type of measurement that has been employed (for example, counts-per-second metric on the ordinate of the relevant plots becomes dimensionless).
The luc reporter was driven by a portion of the period (per) gene (its 5'-flanking sequences and those encoding the N-terminal 2/3 of the protein) in some cases or the 5'-flanking sequences of the timeless (tim) gene in the others; these molecular constructs were introduced into the D. melanogaster genome by germline transformation [ 32 18 33 ] . Reporter activity was sampled from an enzymatic reaction (luciferase oxidizing luciferin, present in the medium surrounding the antennae); the reaction produces a bioluminescent signal that is measured in counts per second once each hour [ 13 ] . One genetically based comparison made in this study involved the effects of the (normal) cry +allele compared with that of cry b, tested in LD 12:12 (12 hours of light followed by 12 hours of darkness, over the course of about 5-6 cycles per specimen).
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