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Example sentences for: bdm

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Post-mitotic cell growth was inhibited by BDM, specifically in the transition zone and the apical parts of the elongation region.
Though BDM does not inhibit nm-myosinII, in permeabilized yeast ( S. cerviseae ) BDM has been shown to decrease incorporation of exogenous actin into the cytoskeleton [ 20 ] . This process of actin incorporation requires proteins involved in actin filament formation and proteins that may localize a nucleation complex [ 20 21 ] . To determine if BDM has a similar effect on actin incorporation in mammalian cells, and if so an effect on actin nucleation, spreading Swiss3T3 cells were treated briefly with a media control or 15 mM BDM and then permeabilized with the detergent saponin in the presence of fluorescently labeled actin.
BDM treatment disrupted normal cellular distributions of maize myosins and the characteristic distribution of F-actin was also affected.
BDM treatment thus seems to disrupt actin dynamics specific to the plasma membrane and this may provide a clue to both the mechanism of BDM inhibition and the relationship of actin dynamics at the plasma membrane.
We found that 20 mM BDM does not affect movement of bacteria inside treated cells [Figure 3A] consistent with a previous report [ 4 ] . Interestingly, localization of Arp3 in Listeria comet tails is unaffected with BDM treatment [Figure 3B] though Arp3 localization at the leading edge of these cells is disrupted.