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Example sentences for: bdm

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An important aspect of actin cytoskeletal research has been the use of small molecules that affect actin dynamics and proteins that act on the cytoskeleton [ 1 ] . 2,3-butanedione monoxime (BDM) is a small molecule that inhibits the ATPase activity of muscle myosinII and reduces the force generated by this motor protein [ 2 3 ] . BDM was introduced to the cell biology community as a millimolar inhibitor of multiple non-muscle myosins with greatest potency in inhibiting non-muscle myosinII [ 4 ] . It was subsequently used, in concert with other approaches, to study nm-myosinII function in a variety of biological processes [ 5 6 7 8 ] . BDM was recently shown not to inhibit any non-muscle myosins so far tested, leaving the question of how it affects cells unanswered [ 9 10 ] .
The ATPase activity of platelet nm-myosinII S1 fragment, is not inhibited by BDM while the inhibitor, blebbistatin, decreases ATPase activity by more than 90%.
BDM has been reported to not inhibit nm-myosinII ATPase activity [ 9 ] . We show data [Figure 1A] that supports this report.
So, is this effect that we see with BDM mediated by other proteins involved in retrograde flow?
After washout of BDM, Arp3-GFP localization returns in just over one minute.