Words similar to bcl

Example sentences for: bcl

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MSCV/syk expression vectors were packaged into viral particles by transient transfection of 1 × 10 6BOSC cells with 15 μg of plasmid DNA/CaPO 4 precipitates [ 61 ] . BCL 1 .3B3 cells were infected with 500 μl of a 1:2 dilution of viral supernatant in growth medium containing 4 μg/ml polybrene (Sigma-Aldrich, Inc.).
Previous studies have implicated Syk in BCR-induced apoptosis in other cell lines [ 37 38 ] . To examine the putative role of Syk in BCR-induced apoptosis and cell cycle arrest in BCL 1 .3B3, we attempted to alter Syk activity through the expression of mutant forms of Syk.
Protein secondary structure was predicted using a multiple alignment as the input for the JPRED and PHD programs [ 42 43 44 ] . Preliminary clustering of proteins was done using the BLASTCLUST program with empirically determined length and score threshold cut off values (For documentation see ftp://ftp.ncbi.nih.gov/blast/documents/README.bcl).
To determine if the constitutive phenotype was a result of the specific mutations introduced or if overexpression of a full-length Syk molecule was sufficient to give high basal kinase activity, Syk activity was analyzed from BCL 1 .3B3 cells that expressed a wildtype human Syk transgene (Figure 6).
First, expression of the (K395A/3F)Syk molecule in BCL 1 .3B3 results in constitutive activation of endogenous Syk.