Words similar to bcip

Example sentences for: bcip

How can you use “bcip” in a sentence? Here are some example sentences to help you improve your vocabulary:

Sequential immunoaffinity columns were used to purify β-tubulin classes II and III with their associated α-tubulins as described previously [ 12 ] . Known amounts of PC-tubulin, β-tubulin classes II and III, and pig brain samples ( n = 12) from the cerebellum and right cerebrum were examined by SDS-polyacrylamide-gel electrophoresis and transferred to PVDF filters [ 43 ] . Alkaline phosphatase colorimetric reactions (NBT and BCIP) were used to identify tubulin isotypes.
The immunolabeled bands were visualized with alkaline-phosphatase conjugated secondary antibody using NBT/BCIP enzyme substrate (Chemicon).
Following nine 10-min washes in 0.1% Tween 20 in PBS and two rinses in AP buffer (50 mM MgCl 2 , 100 mM NaCI, 100 mM Tris pH 9.5, 0.01% Tween 20) the NBT/BCIP color substrates were used to detect the hybridized probes.
For colorimetric detection, hybridization with the targets was revealed by reacting the tissue sections with anti-DIG-alkaline phosphatase conjugate, followed by reaction with nitroblue tetrazolium chloride (NBT), 5-bromo-4-chloro-3-indolyl phosphate (BCIP), and levamisole as previously described [ 39].
Excess secondary antibody was removed by three 5-min washes with TNT buffer and color was developed using the 5-bromo-4-chloro-3-indolylphosphate/nitro blue tetrazolium (BCIP/NBT) alkaline phosphatase substrate.