Example sentences for: bcip

How can you use “bcip” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The membranes were then treated with anti-digoxigenin-AP Fab fragments and the color substrates NBT/BCIP.

  • For colorimetric detection, hybridization with the targets was revealed by reacting the tissue sections with anti-DIG-alkaline phosphatase conjugate, followed by reaction with nitroblue tetrazolium chloride (NBT), 5-bromo-4-chloro-3-indolyl phosphate (BCIP), and levamisole as previously described [ 39].

  • The immunolabeled bands were visualized with alkaline-phosphatase conjugated secondary antibody using NBT/BCIP enzyme substrate (Chemicon).

  • Excess secondary antibody was removed by three 5-min washes with TNT buffer and color was developed using the 5-bromo-4-chloro-3-indolylphosphate/nitro blue tetrazolium (BCIP/NBT) alkaline phosphatase substrate.

  • Nitro blue tetrazolium (NBT), 5-bromo-4-chloroindol-3-yl phosphate (BCIP) and poly(vinylidene difluoride) (PVDF) were from Bio-Rad.


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