Words similar to bcip
Example sentences for: bcip
How can you use “bcip” in a sentence? Here are some example sentences to help you improve your vocabulary:
The membranes were then treated with anti-digoxigenin-AP Fab fragments and the color substrates NBT/BCIP.
For colorimetric detection, hybridization with the targets was revealed by reacting the tissue sections with anti-DIG-alkaline phosphatase conjugate, followed by reaction with nitroblue tetrazolium chloride (NBT), 5-bromo-4-chloro-3-indolyl phosphate (BCIP), and levamisole as previously described [ 39].
BCIP = 5-bromo-4-chloroindol-3-yl phosphate; cDNA = complementary DNA; EGTA = (ethylene-bis [oxyethylenenitrilo])tetraacetic acid; CHTN = Cooperative Human Tissue Network; ELISA = enzyme-linked immunosorbent assay; MAP = microtubule associated protein; MES = 2-( N -morpholino)-ethane sulfonic acid; NBT = nitro blue tetrazolium; PBS = phosphate-buffered saline; PC-tubulin = MAP-free phosphocellulose-purified tubulin; PCR = polymerase chain reaction; PIPES = piperazine- N,N '-bis(2-ethanesulfonic acid); PVDF = poly(vinylidene difluoride).
Sequential immunoaffinity columns were used to purify β-tubulin classes II and III with their associated α-tubulins as described previously [ 12 ] . Known amounts of PC-tubulin, β-tubulin classes II and III, and pig brain samples ( n = 12) from the cerebellum and right cerebrum were examined by SDS-polyacrylamide-gel electrophoresis and transferred to PVDF filters [ 43 ] . Alkaline phosphatase colorimetric reactions (NBT and BCIP) were used to identify tubulin isotypes.
Excess secondary antibody was removed by three 5-min washes with TNT buffer and color was developed using the 5-bromo-4-chloro-3-indolylphosphate/nitro blue tetrazolium (BCIP/NBT) alkaline phosphatase substrate.
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