Example sentences for: bamhi

How can you use “bamhi” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A potential translation initiation codon in the NotI/BamHI fragment (3 kb) of clone 4B1 starts an open reading frame that encodes a polypeptide sequence highly homologous to the first six transmembrane domains of the human EP2 receptor [ 20 ] . The amino acid sequence of the open reading frame in the NotI fragment (6 kb) of clone 6A was homologous to the seventh transmembrane domain (TMD VII), followed by a short C-terminal tail and an in-frame stop codon.

  • Al, 1998) that contained a version of the GFP gene modified to have Xho1 and BamHI sites located between codons specifying N157 and K158, and EcoRI ends subsequently inserted.

  • Promoter 1b was linked to the EGFP-F reporter using a HindIII/BamHI fragment that contained 5' flank, exons 1b and b', 3 KB of intron 1b', and the coding exon splice acceptor site preceded by 250 nucleotides of intron 1c.

  • The PCR product was digested with SacI and XbaI and cloned into the SacI/XbaI sites of both pRS315 (pSW1264) and pRS316 (pSW1268) [ 66 ] . Plasmids with wild type SEC23 were constructed in the same manner using specific oligonucleotides (5' oligo sec23F5 with 5'-ATGAATATCTAGACCAGGGTGCC-3'; 3' oligo sec23-3 with 5'CCTTGGATCCGTAGTAAAGGCCACGCAG-3') and digestion of the PCR product with XbaI and BamHI to yield p SEC23/LEU2 (pSW1266) and p SEC23/URA3 (pSW1269).

  • pΔHprt was constructed by introducing a 2.5 Kb BamHI/HindIII fragment of 5' hprt genomic DNA (E14TG2a origin) from the plasmid pMP8 into the Hpa1 site of pOS.DUP/DEL [ 15 ] . The ends of the fragments were made blunt using Klenow.


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