Words similar to bamhi
Example sentences for: bamhi
How can you use “bamhi” in a sentence? Here are some example sentences to help you improve your vocabulary:
pGST-p20-C/EBPβ was constructed by inserting the 581 bp BamHI/EcoRI fragment from pRsetA-LIP [ 34] into similarly digested pGEX4T-1.
Each population of cDNA was ligated into a pBluescript vector that had been digested with BamHI and alkaline phosphatased to decrease the rate of self-ligation.
To generate Rab24 fusions with Enhanced Green Fluorescent Protein (EGFP), cDNAs encoding full-length Rab24(wt) or Rab24(D123I), or the Rab24 C-terminal hypervariable domain (residues 166-203) were generated with flanking KpnI and BamHI sites and subcloned into pEGFP-C1 (Clontech Inc., Palo Alto, CA) so that the Rab inserts were in-frame with a 5' EGFP coding sequence.
This sequence was then subcloned into the XhoI and BamHI sites of the pET-15b vector by designing similar primers but containing the respective restriction sites.
pΔHprt was constructed by introducing a 2.5 Kb BamHI/HindIII fragment of 5' hprt genomic DNA (E14TG2a origin) from the plasmid pMP8 into the Hpa1 site of pOS.DUP/DEL [ 15 ] . The ends of the fragments were made blunt using Klenow.
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