Words similar to bamhi

Example sentences for: bamhi

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To generate Rab24 fusions with Enhanced Green Fluorescent Protein (EGFP), cDNAs encoding full-length Rab24(wt) or Rab24(D123I), or the Rab24 C-terminal hypervariable domain (residues 166-203) were generated with flanking KpnI and BamHI sites and subcloned into pEGFP-C1 (Clontech Inc., Palo Alto, CA) so that the Rab inserts were in-frame with a 5' EGFP coding sequence.
pΔHprt was constructed by introducing a 2.5 Kb BamHI/HindIII fragment of 5' hprt genomic DNA (E14TG2a origin) from the plasmid pMP8 into the Hpa1 site of pOS.DUP/DEL [ 15 ] . The ends of the fragments were made blunt using Klenow.
The PCR product was cloned into the BamHI site of pBP3:ER (pBabepuro containing the hormone-binding domain of the HE14 allele of the human estrogen receptor) [ 26].
The 3' arm of homology was introduced into the unique Bcl1 site of the previous plasmid as a 7 Kb BamHI fragment from pMP1(SV129 origin) that contained murine hprt exons 2 and 3.
pVT560 was constructed by first filling the BamHI and XhoI sites in pEG202 (Gyuris et.