Example sentences for: bamhi

How can you use “bamhi” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • [ 64 ] . To create a template for the polymerase chain reaction (PCR), the BamHI fragment of GFP S65T F64L [ 65 ] was first cloned into pRS316 [ 66 ] . The DNA fragment for integration was generated by PCR using the following oligonucleotides: Nup170G (5'-GATCCAATTGAAAAGTACGTTAAGAACAGCGGCAATAATTTGGGGATTTGTTTCTACAAAGAAGCTGCGATGAGTAAAGGAGAAGAACTT-3') and Nup170V (5'-TTACTTACAGATTACGATCTGTTCAATGCATCAGAAATAGGAAGAAATTCAAAGCGGCATCAGAGCAGATTGT-3').

  • Each population of cDNA was ligated into a pBluescript vector that had been digested with BamHI and alkaline phosphatased to decrease the rate of self-ligation.

  • A potential translation initiation codon in the NotI/BamHI fragment (3 kb) of clone 4B1 starts an open reading frame that encodes a polypeptide sequence highly homologous to the first six transmembrane domains of the human EP2 receptor [ 20 ] . The amino acid sequence of the open reading frame in the NotI fragment (6 kb) of clone 6A was homologous to the seventh transmembrane domain (TMD VII), followed by a short C-terminal tail and an in-frame stop codon.

  • pGST-p20-C/EBPβ was constructed by inserting the 581 bp BamHI/EcoRI fragment from pRsetA-LIP [ 34] into similarly digested pGEX4T-1.

  • The PCR product was cloned into the BamHI site of pBP3:ER (pBabepuro containing the hormone-binding domain of the HE14 allele of the human estrogen receptor) [ 26].


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