Words similar to bamhi
Example sentences for: bamhi
How can you use “bamhi” in a sentence? Here are some example sentences to help you improve your vocabulary:
[ 64 ] . To create a template for the polymerase chain reaction (PCR), the BamHI fragment of GFP S65T F64L [ 65 ] was first cloned into pRS316 [ 66 ] . The DNA fragment for integration was generated by PCR using the following oligonucleotides: Nup170G (5'-GATCCAATTGAAAAGTACGTTAAGAACAGCGGCAATAATTTGGGGATTTGTTTCTACAAAGAAGCTGCGATGAGTAAAGGAGAAGAACTT-3') and Nup170V (5'-TTACTTACAGATTACGATCTGTTCAATGCATCAGAAATAGGAAGAAATTCAAAGCGGCATCAGAGCAGATTGT-3').
pVT560 was constructed by first filling the BamHI and XhoI sites in pEG202 (Gyuris et.
Each population of cDNA was ligated into a pBluescript vector that had been digested with BamHI and alkaline phosphatased to decrease the rate of self-ligation.
We used genomic DNA for a template, and for each mutant transcribed the complete ftsZ sequence (adding NdeI and BamHI cloning sites at the ends) using error-correcting Pfu polymerase [ 37] and outward-facing primers containing the desired mutations.
Promoter 1b was linked to the EGFP-F reporter using a HindIII/BamHI fragment that contained 5' flank, exons 1b and b', 3 KB of intron 1b', and the coding exon splice acceptor site preceded by 250 nucleotides of intron 1c.
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