Words similar to bamhi
Example sentences for: bamhi
How can you use “bamhi” in a sentence? Here are some example sentences to help you improve your vocabulary:
[ 64 ] . To create a template for the polymerase chain reaction (PCR), the BamHI fragment of GFP S65T F64L [ 65 ] was first cloned into pRS316 [ 66 ] . The DNA fragment for integration was generated by PCR using the following oligonucleotides: Nup170G (5'-GATCCAATTGAAAAGTACGTTAAGAACAGCGGCAATAATTTGGGGATTTGTTTCTACAAAGAAGCTGCGATGAGTAAAGGAGAAGAACTT-3') and Nup170V (5'-TTACTTACAGATTACGATCTGTTCAATGCATCAGAAATAGGAAGAAATTCAAAGCGGCATCAGAGCAGATTGT-3').
pEGFP-N280 was digested with EcoRI and BamHI, filled in with Klenow DNA polymerase on both ends, and re-ligated to generate pEGFP-N200.
To construct the cTOP1 expression vector (pDBleu-TOP1c), cTOP1 was excised from pGBD-cTOP1 with EcoRI and BamHI and inserted into the same sites in pDBleu (ProQuest, Life Technologies).
pΔHprt was constructed by introducing a 2.5 Kb BamHI/HindIII fragment of 5' hprt genomic DNA (E14TG2a origin) from the plasmid pMP8 into the Hpa1 site of pOS.DUP/DEL [ 15 ] . The ends of the fragments were made blunt using Klenow.
pVT560 was constructed by first filling the BamHI and XhoI sites in pEG202 (Gyuris et.
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