Words similar to bam

Example sentences for: bam

How can you use “bam” in a sentence? Here are some example sentences to help you improve your vocabulary:

To generate pGa14-m.nocturnin, a fragment from the m.nocturnin cDNA [ 21 ] corresponding to the entire coding sequence (with 9 amino acids upstream of the initiation codon) was excised from pGEX-3X upon Sma I and Dra I digestion, and was inserted into the pGaL4PolyII vector opened by Bam Hl, after Klenow treatment of both vector and insert.
The 5' and 3' primers contained Nde I and Bam HI restriction sites, respectively, and were designed to amplify the rec12 +cDNA from the first ATG in exon 1 to a position +142 bp downstream of the stop codon in exon 5. RT-PCR products were cloned by blunt-end ligation into pCR-Blunt (Invitrogen Corp.) and both strands were subject to DNA sequencing (GenBank accession no.
This PCR fragment was cloned into the Bam HI site of pBPhin [ 31 ] to generate pHIP.
An Nde I- Bam HI fragment of a cDNA clone with wild-type rec12 +sequence (see above) was sub-cloned into the pREP42 vector to generate pREP42- rec12 + cDNA . The integrity of all clones was determined by a combination of restriction mapping, PCR, and DNA sequencing.
Plasmid pHIP14 was created by cloning a Bam HI - digested PCR fragment containing Rv2238c, Rv2239c, and Rv2240c into the Bam HI site downstream of the hsp65 promoter of pHIP such that Rv2240c was proximal to the hsp65 promoter.