Words similar to bam

Example sentences for: bam

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An amplicon carrying the complete xylE ORF without the xylE promoter was generated by PCR of pTKmx [ 32 ] , cleaved with Bam HI, and cloned into the Bam HI site of pHIP downstream from the hsp65 promoter to generate pHIP1.
The intron-less pce1 +gene was amplified from plasmid pl32-PCE1 [ 12 ] . The PCR products were digested with Xho I and Bam HI and then inserted into the S. pombe expression vector pREP41X ( LEU2 ars1 +) [ 19 ] . The inserts were sequenced to exclude the acquisition of unwanted mutations during the amplification and cloning steps.
Each intended mutation was confirmed by DNA sequence analysis [ 34 ] . A Hinc II/ Bam HI fragment containing the regulatory region was excised from the M13 template and inserted into the plasmid pRS1274 to give the JA250 (wild type) and related mutant dmsA - lac Z promoter fusions (Figure 1).
Dengue-2 virus cDNA cloned in the yeast shuttle vector pRS424, linearized by excision of a short Bam H1 fragment was transfected into competent [ 45 ] S. cerevisiae YPH857 (kindly provided by Barry Falgout, (CBER/FDA), along with the appropriate PCR fragment spanning the desired deletion.
The Bam HI/ Eco RI fragment of the M. tuberculosis H37Rv insert of the recovered plasmid was used as a probe in colony blots to find cosmids in the E. coli (pYUB178::H37Rv) library.