Words similar to bam

Example sentences for: bam

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The PCR product was digested with Eco RI and Bam HI and ligated into the Eco RI and Bam HI restriction sites of pGBT9 to produce construct pGBT9/prodelHB-EGF.
Dengue-2 virus cDNA cloned in the yeast shuttle vector pRS424, linearized by excision of a short Bam H1 fragment was transfected into competent [ 45 ] S. cerevisiae YPH857 (kindly provided by Barry Falgout, (CBER/FDA), along with the appropriate PCR fragment spanning the desired deletion.
URA3 was amplified as a ~ 1.1 kb fragment from pRS306 [ 32 ] . Before TAR cloning, the vector was linearized with Bam HI to expose the B1 targeting sequence and the URA3 sequence placed at the end of the transgene-specific hook.
A cosmid corresponding to the one in the sur3 clone was isolated from the E. coli (pYUB178::H37Rv) library by probing colony blots with the 4.5 kb Bam HI/ Eco Rl fragment of the M. tuberculosis genomic DNA insert in the plasmid recovered from the sur3 clone.
Nde I and Bam HI restriction sites of the inducible vectors pREP2, pREP42, and pREP82 [ 32 ] . Induction of gene expression and northern blot analysis of rec12 +mRNA levels were as described [ 32 57 ] .