Words similar to bamh
Example sentences for: bamh
How can you use “bamh” in a sentence? Here are some example sentences to help you improve your vocabulary:
Fig 1cshows that digestion of wild-type R1 ES cell DNA with BamH1 did indeed generate a 7.0 kb fragment that could be detected with intron 3 probe.
For the yeast two-hybrid screen, full-length Smad1 was subcloned into BamH1/Not1 sites of PEG202 by a PCR method, resulting in the inframe fusion between the LexA DNA binding domain (1-202) and Smad1.
In contrast, F3 cells were shown to have replaced this fragment with an 8.3 kb BamH1 fragment thereby confirming the integrity of the targeting event.
To search for additional large genomic rearrangements, we carried out Southern analyses of BamH I, Sac I and Bgl II digested genomic DNA from 18 fibroblasts strains that were negative for FBN1 mutation screening by other methods, but had shown abnormal fibrillin biosynthetic profiles by pulse-chase analysis.
PCR products were restriction digested with BamH1 (exon 1) or Not1 (exon 2), purified from an agarose gel by Qiaquick extraction (Qiagen), and simultaneously subcloned into a BamH1/Not1 digested pcDNA3 (Invitrogen) vector to create a directional clone of rabbit MYOC exons 1 and 2 spliced together (pcDNA3.RbMYOCex1-2).