Example sentences for: bamh

How can you use “bamh” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • PCR products were restriction digested with BamH1 (exon 1) or Not1 (exon 2), purified from an agarose gel by Qiaquick extraction (Qiagen), and simultaneously subcloned into a BamH1/Not1 digested pcDNA3 (Invitrogen) vector to create a directional clone of rabbit MYOC exons 1 and 2 spliced together (pcDNA3.RbMYOCex1-2).

  • Human wild type p53 or p53 bearing mutations in serines 33 or 37 were inserted into the BamH1 site of the pcDNA3.

  • For the yeast two-hybrid screen, full-length Smad1 was subcloned into BamH1/Not1 sites of PEG202 by a PCR method, resulting in the inframe fusion between the LexA DNA binding domain (1-202) and Smad1.

  • The PCR product was then cloned into the pET15b vector which carries an N-terminal Histag sequence followed by BamH1 and Nde 1 cloning sites (Novagen Inc., Madison, WI).

  • As shown in fig 1athe correct integration of Neo at the hprt locus was predicted to delete a BamH1 restriction endonuclease cut-site from intron 1 and replace it with a BamH1 site that is unique to the mutant hprt allele.


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