Words similar to bamh
Example sentences for: bamh
How can you use “bamh” in a sentence? Here are some example sentences to help you improve your vocabulary:
Human wild type p53 or p53 bearing mutations in serines 33 or 37 were inserted into the BamH1 site of the pcDNA3.
PCR products were restriction digested with BamH1 (exon 1) or Not1 (exon 2), purified from an agarose gel by Qiaquick extraction (Qiagen), and simultaneously subcloned into a BamH1/Not1 digested pcDNA3 (Invitrogen) vector to create a directional clone of rabbit MYOC exons 1 and 2 spliced together (pcDNA3.RbMYOCex1-2).
To construct the expression plasmid encoding C-terminal-myc-tagged truncated BTBD2 having the N-terminal half the BTB domain deleted (m-BTBD2T), the coding region of BTBD2 from the second ATG to almost the end of the 3'-UTR (445-2542 bp) was amplified with primers (Upper primer: 5'-ATGTTCAACGGGGGAATGGCCACAACATC-3', Lower primer: 5'-GGGGAGGACGCAGGTTCCAGGAGT-3') containing BamH I and Hind III cleavage sites and inserted into pCMV-Tag 3 vector (Stratagene) cut with BamH I and Hind III.
To search for additional large genomic rearrangements, we carried out Southern analyses of BamH I, Sac I and Bgl II digested genomic DNA from 18 fibroblasts strains that were negative for FBN1 mutation screening by other methods, but had shown abnormal fibrillin biosynthetic profiles by pulse-chase analysis.
Fig 1cshows that digestion of wild-type R1 ES cell DNA with BamH1 did indeed generate a 7.0 kb fragment that could be detected with intron 3 probe.