Words similar to atg
Example sentences for: atg
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The following conditions are thought to be criteria for the ribosomal scanning mechanism: an absence of any ATG codons in the intercistronic region, an intercistronic region of 15 to 78 bp, and an optimized consensus translation start site for the second CDS.
Both selected targeting sequences do not contain an ATG initiation codon that is out-of-frame with the vector URA3 , which avoids the translational incompetence of URA3 mRNA and resistance to 5-FOA (see Methods).
The CaCET1 gene was disrupted by insertion of a UAU1 cassette [ 16 ] . We first constructed plasmid pKS-5'3'CaCET1, in which a 665-bp PCR fragment derived from the 5' end of the CaCET1 gene (from nucleotides -50 to +615 of the open reading frame, with the A residue of the ATG translation start codon defined as position +1) was cloned between the Kpn I and Xba I sites of pBluescript KS +and a 720-bp fragment extending from position +1518 of the 1560-nt CaCET1 coding sequence into the 3' flanking genomic region was inserted between the Sac I and Sac II sites of pBluescript KS +The 3.8-kbp UAU1 gene was excised from pBME101 with Xba I and Sac II and inserted between the Xba I and Sac II sites of pKS-5'3'CaCET1 to yield pCaCET1::UAU1.
We mutated an out-of-frame ATG codon identified in a B1 template sequence during PCR amplification of the hook to avoid translational incompetence and resistance to 5-FOA.
This prediction is based on the occurrence of three in-frame stop codons upstream of the indicated ATG, within the 242 nt 5'-untranslated region (5'-UTR) (see Additional File 1).
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