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Example sentences for: arg
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To examine a wider range of promoter and polymerase combinations, a promoter library was constructed in which positions -8 through -11 in the promoter were completely randomized; each of these nucleotides had previously been shown to be extremely important for the specificity of interactions with T7 RNA polymerase [ 6 7 9 11 ] . Conversely, residues Asn 748, Arg 756, and Gln 758 were known to form sequence specific contacts with this region of the promoter [ 2 ] . The promoter library was combined with the gene library in order to create approximately 51,200 (4 4× 20 3) combinations of promoters and polymerases.
The deleted segment contains the catalytic domain essential for triphosphatase activity in vitro and for complementation of the cet1Δ strain of S. cerevisiae [ 10 11 ] . Second, we introduced the linearized deletion allele into a diploid C. albicans ura3/ura3 arg4/arg4 strain and selected for Arg +transformants.
The 2.7-kbp fragment was also detected with an ARG4 -specific probe (not shown).
The P1 (Arg346) residue in the RCL of PAI-1 is an essential determinant for its target specificity and inhibitory activity.
A single T-to-G transversion at mtDNA position 8,993 changes leucine 156 of ATP synthase subunit 6 to arginine (Leu56 → Arg) [ 14] (Figure 4a).