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Example sentences for: aptazymes

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Design of Group I aptazymes
These results argue that both GTP and theophylline may need to be present to influence the folding pathway of the engineered Group I aptazymes.
This would seem especially true in the current set of experiments, since the modulation of Thymidylate Synthase expression is known to greatly affect thymidine levels and cell growth [ 32 ] . For example, E. coli that are starved for thymine quickly die, a phenomenon known as 'thymineless death' [ 33 ] . In this respect, Group I aptazymes that may have yielded lower amounts of functional Thymidylate Synthase (e.g.
Adjoining aptamers to the Group I self-splicing intron would potentially allow the control of splicing not only in vitro , but in vivo . To this end, we have attempted to generate and assay Group I aptazymes.
For all of the aptazymes except Th3P6, the ligand-induced splicing activity was greater in a standard assay at the more stringent (3 mM) magnesium concentration (Table 1).