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Example sentences for: aptazymes
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Design of Group I aptazymes
The self-splicing activities of the Group I aptazymes were examined in vitro using a standard splicing assay (Table 1).
Group I aptazymes that were dependent upon theophylline both in vitro and in vivo were engineered by conjoining the thymidylate synthase ( td ) intron with an anti-theophylline aptamer.
In order to examine effector-specificity in vivo , a new series of Group I aptazymes was constructed in which the anti-theophylline aptamer was mutated to bind 3-methylxanthine (3MeX2P6) [ 34 ] . These variants proved to be responsive to 3-methylxanthine both in vitro and in vivo (Figure 6a).
These results argue that both GTP and theophylline may need to be present to influence the folding pathway of the engineered Group I aptazymes.
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